Team:TU Darmstadt/protocols/Chemically competent cells
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!align="center"|[[Team:TU_Darmstadt/materials|<span style="color:white;font-size:160%;"> Materials |</span>]] | !align="center"|[[Team:TU_Darmstadt/materials|<span style="color:white;font-size:160%;"> Materials |</span>]] | ||
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+ | == SOC == | ||
+ | SOC medium is used in [https://2012.igem.org/Team:TU_Darmstadt/protocols/Bacterial_transformation bacterial transformation]. | ||
+ | |||
+ | === Ingredients === | ||
+ | *[https://2012.igem.org/Team:TU_Darmstadt/materials/SOB SOB] | ||
+ | *20 mM glucose | ||
+ | |||
+ | ===Protocol=== | ||
+ | #Make 1 L of [https://2012.igem.org/Team:TU_Darmstadt/materials/SOB SOB] media | ||
+ | #After autoclaving and cooling to less than 50°C, add 20 ml filter sterilized 20% glucose solution |
Revision as of 16:57, 1 October 2013
Labbook | | Protocols | | Materials | |
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SOC
SOC medium is used in bacterial transformation.
Ingredients
- SOB
- 20 mM glucose
Protocol
- Make 1 L of SOB media
- After autoclaving and cooling to less than 50°C, add 20 ml filter sterilized 20% glucose solution