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Team:TU Darmstadt/protocols/Chemically competent cells
From 2013.igem.org
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== SOC == | == SOC == | ||
| - | SOC medium is used in [https:// | + | SOC medium is used in [https://2013.igem.org/Team:TU_Darmstadt/protocols/Heat_Shock_Transformation bacterial transformation]. |
=== Ingredients === | === Ingredients === | ||
Revision as of 17:15, 1 October 2013
| Labbook | | Protocols | | Materials | |
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SOC
SOC medium is used in bacterial transformation.
Ingredients
- SOB
- 20 mM glucose
Protocol
- Make 1 L of SOB media
- After autoclaving and cooling to less than 50°C, add 20 ml filter sterilized 20% glucose solution
