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Team:TU Darmstadt/protocols/Chemically competent cells
From 2013.igem.org
(Difference between revisions)
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=== Ingredients === | === Ingredients === | ||
| - | *[https:// | + | *[https://2013.igem.org/Team:TU_Darmstadt/materials/SOB SOB] |
*20 mM glucose | *20 mM glucose | ||
===Protocol=== | ===Protocol=== | ||
| - | #Make 1 L of [https:// | + | #Make 1 L of [https://2013.igem.org/Team:TU_Darmstadt/materials/SOB SOB] media |
#After autoclaving and cooling to less than 50°C, add 20 ml filter sterilized 20% glucose solution | #After autoclaving and cooling to less than 50°C, add 20 ml filter sterilized 20% glucose solution | ||
Revision as of 17:13, 1 October 2013
| Labbook | | Protocols | | Materials | |
|---|
SOC
SOC medium is used in bacterial transformation.
Ingredients
- SOB
- 20 mM glucose
Protocol
- Make 1 L of SOB media
- After autoclaving and cooling to less than 50°C, add 20 ml filter sterilized 20% glucose solution
