Team:TU Darmstadt/protocols/Chemically competent cells
From 2013.igem.org
Labbook | | Protocols | | Materials | |
---|
SOC
SOC medium is used in bacterial transformation.
Ingredients
- SOB
- 20 mM glucose
Protocol
- Make 1 L of SOB media
- After autoclaving and cooling to less than 50°C, add 20 ml filter sterilized 20% glucose solution