Team:TU Darmstadt/protocols/Chemically competent cells

From 2013.igem.org

Revision as of 17:13, 1 October 2013 by Schiefie (Talk | contribs)

Labbook | Protocols | Materials |

SOC

SOC medium is used in bacterial transformation.

Ingredients

  • SOB
  • 20 mM glucose

Protocol

  1. Make 1 L of SOB media
  2. After autoclaving and cooling to less than 50°C, add 20 ml filter sterilized 20% glucose solution