Team:TU Darmstadt/protocols/InFusion

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<B> Materials<br></B>
<B> Materials<br></B>
 +
5X In-Fusion HD Enzyme Premix
 +
<br>pUC19 Control Vector,linearized (50 ng/μl)
 +
<br>2 kb Control Insert (40 ng/μl)<br>
<br>
<br>
<B> Equipment<br></B>
<B> Equipment<br></B>

Revision as of 17:40, 4 October 2013





In Fusion

Materials
5X In-Fusion HD Enzyme Premix
pUC19 Control Vector,linearized (50 ng/μl)
2 kb Control Insert (40 ng/μl)

Equipment
In-Fusion® HD Cloning Kit
Micropipettes with sterile tips

Chemicals & consumables



Procedure
1.
2.
3.
4.