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Team:TU Darmstadt/protocols/PCR
From 2013.igem.org
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This is a commonly used protocol thta we used for Taq-polymerase<br> | This is a commonly used protocol thta we used for Taq-polymerase<br> | ||
<B>PCR program (30 cycles)<br></B> | <B>PCR program (30 cycles)<br></B> | ||
| - | initial denaturation 95°C, 60s | + | initial denaturation 95°C, 60s<br> |
| - | denaturation 95°C, 45s | + | denaturation 95°C, 45s<br> |
| - | annealing 54,5°C, 90s | + | annealing 54,5°C, 90s <br> |
| - | elongation 72°C, 120s | + | elongation 72°C, 120s<br> |
| - | final elongation 72°C, 120s | + | final elongation 72°C, 120s<br> |
| - | + | <br> | |
After worth run an analytic 1% agarose gel and purificate the product with Wizard SV Gel and PCR Clean-Up System (Promega) | After worth run an analytic 1% agarose gel and purificate the product with Wizard SV Gel and PCR Clean-Up System (Promega) | ||
<br> | <br> | ||
Revision as of 16:44, 4 October 2013
PCR
Short report
Materials
Equipment
Micropipettes with sterile tips
PCR machine
PCR tubes
Chemicals & consumables
DNA template
Nuclease-free water
Primer
Polymerase buffer
dNTP mix
DMSO
Procedure
This is a commonly used protocol thta we used for Taq-polymerase
PCR program (30 cycles)
initial denaturation 95°C, 60s
denaturation 95°C, 45s
annealing 54,5°C, 90s
elongation 72°C, 120s
final elongation 72°C, 120s
After worth run an analytic 1% agarose gel and purificate the product with Wizard SV Gel and PCR Clean-Up System (Promega)
