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Team:TU Darmstadt/protocols/PCR
From 2013.igem.org
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| + | <a href="https://2013.igem.org/Team:TU_Darmstadt/labbook"> | ||
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| + | <font size="8" color="#F0F8FF" face="Arial regular">Materials |</font> | ||
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| + | <a href="https://2013.igem.org/Team:TU_Darmstadt/protocols"> | ||
| + | <font size="8" color="#F0F8FF" face="Arial regular">Protocols</font> | ||
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<h2><font size="6" color="#F0F8FF" face="Arial regular"> PCR </font></h2> | <h2><font size="6" color="#F0F8FF" face="Arial regular"> PCR </font></h2> | ||
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Latest revision as of 00:37, 5 October 2013
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PCR
Short report
PCR (polymerase chain reaction) is a method for exponentially amplifying a fragment of DNA in vitro.
Materials
Equipment
Micropipettes with sterile tips
PCR machine
PCR tubes
Chemicals & consumables
DNA template
Nuclease-free water
Primer
Polymerase buffer
dNTP mix
DMSO
Procedure
This is a commonly used protocol thta we used for Taq-polymerase
PCR program (30 cycles)
initial denaturation 95°C, 60s
denaturation 95°C, 45s
annealing 54,5°C, 90s
elongation 72°C, 120s
final elongation 72°C, 120s
After worth run an analytic 1% agarose gel and purificate the product with Wizard SV Gel and PCR Clean-Up System (Promega)
