Team:Tianjin/Data

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Contents

BioBrick


Favorite Tianjin 2013 iGEM Team Parts

Name Type Description Designer Length
Bba_K1020005 Composite Alkane Marker--Device for detection of various alkanes Boxuan Zeng
Yue Liu
2146bp
Bba_K1020012 Composite Alkane Selector for intracellular alkane production Boxuan Zeng
Yue Liu
2662bp


Tianjin 2013 iGEM Team Parts Sandbox

Name Type Description Designer Length
Bba_K1020001 Reporter PalkM promoter with reporter gene Boxuan Zeng
Yue Liu
1013bp
Bba_K1020002 Coding alkane regulatory protein ALKR Boxuan Zeng 927bp
Bba_K1020003 Reporter AlkR -PSB1C3-Low constitutive Yue Liu 1125bp
Bba_K1020004 Composite Biosensor for alkane detection Yue Liu
Su Wang
2009bp
Bba_K1020006 Coding YbhGFSR Boxuan Zeng
Yang Wu
4971bp
Bba_K1020007 Composite AlkR -PSB1C3-High constitutive Yue Liu 1125bp
Bba_K1020008 Composite alkane producing module Boxuan Zeng
Yue Liu
1809bp
Bba_K1020009 Composite YbhGFSR operon Boxuan Zeng 5034bp
Bba_K1020010 Coding TetA gene under the control of PalkM Boxuan Zeng
Shoujie Sun
1529bp
Bba_K1020011 Composite High expressed Alkane Selector for intracellular alkane production Boxuan Zeng
Qingyi Deng
2662bp

Others


Results for Agarose Gel Electrophoresis:

gel 1

Figure 1. AlkR-1 ~600bp cut from AlkR-1-pEASY-BLUNT


gel 2

Figure 2. AlkR-2 ~600bp cut from AlkR-2-pEASY-BLUNT

gel 3

Figure 3. J23100/J23103-AlkR-tt-RFP ~2000bp cut from J23100/J23103-AlkR-tt-RFP-pEASY-BLUNT

gel 4

Figure 4. J23100/J23103-AlkR-tt-PalkM-RFP ~2000bp cut from J23100/J23103-AlkR-tt-PalkM-RFP-pSB1C3


gel 5

Figure 5. J23100/J23103-AlkR-tt-PalkM-tetA ~2400bp cut from J23100/J23103-AlkR-tt-PalkM-tetA-pSB1C3

gel 6

Figure 6. J23100/J23114-NPDC-AAR ~2000bp cut from J23100/J23114-NPDC-AAR-pSB3K3

Characterization for our construction:


Figure 7. The visible color change of the strain cultured in 3ML M9 medium with different induced for 12 hours.
Figure 8. Strain one contains alkane producer part(left),another contains none-producer part (right) .

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