http://2013.igem.org/wiki/index.php?title=Team:Tianjin/Project/Background&feed=atom&action=historyTeam:Tianjin/Project/Background - Revision history2024-03-29T05:01:30ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:Tianjin/Project/Background&diff=358686&oldid=prevXIAOKE: Blanked the page2013-10-29T02:26:18Z<p>Blanked the page</p>
<a href="http://2013.igem.org/wiki/index.php?title=Team:Tianjin/Project/Background&diff=358686&oldid=326691">Show changes</a>XIAOKEhttp://2013.igem.org/wiki/index.php?title=Team:Tianjin/Project/Background&diff=326691&oldid=prevYnzh219: /* 2. Biosynthesis of alkanes */2013-10-24T14:32:57Z<p><span class="autocomment">2. Biosynthesis of alkanes</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p> Pathways of long-chain alkane sythesis in microbes has been studied. Two enzymes, acyl-ACP reductase(AAR) and aldehyde decarbonylase(ADC), were heterologously expressed in E.coli to reduce fatty acyl-ACPs to corresponding aldehydes and then convert them to alkanes[3]. Fatty aldehydes can also be produced from fatty acids and fatty acyl-CoAs, catalyzed by acyl-CoA reductases(ACR<del class="diffchange diffchange-inline">) and carboxylic acid reductase(CAR</del>) respectively, which <del class="diffchange diffchange-inline">have </del>been identified in many species[4,5].</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p> Pathways of long-chain alkane sythesis in microbes has been studied. Two enzymes, acyl-ACP reductase(AAR) and aldehyde decarbonylase(ADC), were heterologously expressed in E.coli to reduce fatty acyl-ACPs to corresponding aldehydes and then convert them to alkanes[3]. Fatty aldehydes can also be produced from fatty acids and fatty acyl-CoAs, catalyzed by <ins class="diffchange diffchange-inline">carboxylic acid reductase(CAR) and </ins>acyl-CoA reductases(ACR) respectively, which <ins class="diffchange diffchange-inline">has </ins>been identified in many species[4,5].</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="text-align:center;vertical-align:middle;"><a href="https://static.igem.org/mediawiki/2013/d/dc/Tju-bg-new-01.png" target="_blank" ><img src="https://static.igem.org/mediawiki/2013/d/dc/Tju-bg-new-01.png" width="500px" /></a></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="text-align:center;vertical-align:middle;"><a href="https://static.igem.org/mediawiki/2013/d/dc/Tju-bg-new-01.png" target="_blank" ><img src="https://static.igem.org/mediawiki/2013/d/dc/Tju-bg-new-01.png" width="500px" /></a></div></div></td></tr>
</table>Ynzh219http://2013.igem.org/wiki/index.php?title=Team:Tianjin/Project/Background&diff=326689&oldid=prevYnzh219: /* 1. Call for Biofuels, especially Alkanes */2013-10-24T14:31:56Z<p><span class="autocomment">1. Call for Biofuels, especially Alkanes</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Among all kinds of biofuels, alkanes stand out because of their excellent properties. First, alkanes have higher energy density, for example, enthalpy of combustion of pentadecane is approximately -47.0 MJ/kg compared with -29.7 MJ/kg for ethanol [2]. Then, compared with ethanols with a freezing point of -114℃,which alkanes have a higher freezing point of about -3~19℃, so they are more likely to be compatible with existing engines as well as transport and storage <del class="diffchange diffchange-inline">infrastructure</del>. Besides, they can serve as drop-in replacement for fossil fuels.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Among all kinds of biofuels, alkanes stand out because of their excellent properties. First, alkanes have higher energy density, for example, enthalpy of combustion of pentadecane is approximately -47.0 MJ/kg compared with -29.7 MJ/kg for ethanol [2]. Then, compared with ethanols with a freezing point of -114℃,which alkanes have a higher freezing point of about -3~19℃, so they are more likely to be compatible with existing engines as well as transport and storage <ins class="diffchange diffchange-inline">infrastructures</ins>. Besides, they can serve as drop-in replacement for fossil fuels.</p></div></td></tr>
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</table>Ynzh219http://2013.igem.org/wiki/index.php?title=Team:Tianjin/Project/Background&diff=326685&oldid=prevYnzh219: /* 1. Call for Biofuels, especially Alkanes */2013-10-24T14:28:04Z<p><span class="autocomment">1. Call for Biofuels, especially Alkanes</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p> Nowadays, scientist have put great <del class="diffchange diffchange-inline">attentions </del>on biofuels, hoping to find a solution to energy crisis and climate change. Compared with fossil fuels, biofuels are renewable, and biofuel can be used indefinitely without any net carbon emissions [1]. Therefore, biofuels are promising candidates for mitigating dependence on diesel fuels.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p> Nowadays, scientist have put great <ins class="diffchange diffchange-inline">attention </ins>on biofuels, hoping to find a solution to energy crisis and climate change. Compared with fossil fuels, biofuels are renewable, and biofuel can be used indefinitely without any net carbon emissions [1]. Therefore, biofuels are promising candidates for mitigating dependence on diesel fuels.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Among all kinds of biofuels, alkanes <del class="diffchange diffchange-inline">stands </del>out because of their excellent properties. First, alkanes have higher energy density, for example, enthalpy of combustion of pentadecane is approximately -47.0 MJ/kg compared with -29.7 MJ/kg for ethanol [2]. Then, compared with ethanols with a freezing point of -114℃,which alkanes have a higher freezing point of about -3~19℃, so they are more likely to be compatible with existing engines as well as transport and storage infrastructure. Besides, they can serve as drop-in replacement for fossil fuels.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Among all kinds of biofuels, alkanes <ins class="diffchange diffchange-inline">stand </ins>out because of their excellent properties. First, alkanes have higher energy density, for example, enthalpy of combustion of pentadecane is approximately -47.0 MJ/kg compared with -29.7 MJ/kg for ethanol [2]. Then, compared with ethanols with a freezing point of -114℃,which alkanes have a higher freezing point of about -3~19℃, so they are more likely to be compatible with existing engines as well as transport and storage infrastructure. Besides, they can serve as drop-in replacement for fossil fuels.</p></div></td></tr>
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</table>Ynzh219http://2013.igem.org/wiki/index.php?title=Team:Tianjin/Project/Background&diff=324623&oldid=prevXIAOKE: /* 1. Call for Biofuels, especially Alkanes */2013-10-22T15:25:25Z<p><span class="autocomment">1. Call for Biofuels, especially Alkanes</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p> Nowadays, scientist have put great attentions on biofuels, hoping to find a solution to energy crisis and climate change. Compared with fossil fuels, biofuels are renewable, and biofuel can be used indefinitely without any net carbon emissions [1]. Therefore, biofuels are promising candidates for mitigating dependence on diesel fuels.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p> Nowadays, scientist have put great attentions on biofuels, hoping to find a solution to energy crisis and climate change. Compared with fossil fuels, biofuels are renewable, and biofuel can be used indefinitely without any net carbon emissions [1]. Therefore, biofuels are promising candidates for mitigating dependence on diesel fuels.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Figure 1.</b>&nbsp; Comparison of carbon cycles of fossil fuels and biofuels </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Figure 1.</b>&nbsp; Comparison of carbon cycles of fossil fuels and biofuels </div></div></td></tr>
</table>XIAOKEhttp://2013.igem.org/wiki/index.php?title=Team:Tianjin/Project/Background&diff=324607&oldid=prevXIAOKE: /* 3. Sensing & Detecting alkanes */2013-10-22T15:21:18Z<p><span class="autocomment">3. Sensing & Detecting alkanes</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>However, current methods of alkane detection have many limits. First, extracting the product from original samples and operating high-tech equipments such as GC-MS makes the analysis process quite costly, time-consuming and laborious. Second, to select out target strains in directed evolution, commonly used screening tools are inherently low throughput. What's more, the current methods can hardly perform real-time in vivo detection for industrial production. We’re looking forward to developing an alkane sensor without these limits.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>However, current methods of alkane detection have many limits. First, extracting the product from original samples and operating high-tech equipments such as GC-MS makes the analysis process quite costly, time-consuming and laborious. Second, to select out target strains in directed evolution, commonly used screening tools are inherently low throughput. What's more, the current methods can hardly perform real-time in vivo detection for industrial production. We’re looking forward to developing an alkane sensor without these limits.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="text-align:center;"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="text-align:center;"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Figure 3.</b>&nbsp; GC-MS, a high-tech equipment often used in alkane detection </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Figure 3.</b>&nbsp; GC-MS, a high-tech equipment often used in alkane detection </div></div></td></tr>
</table>XIAOKEhttp://2013.igem.org/wiki/index.php?title=Team:Tianjin/Project/Background&diff=324604&oldid=prevXIAOKE: /* 2. Biosynthesis of alkanes */2013-10-22T15:20:34Z<p><span class="autocomment">2. Biosynthesis of alkanes</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p> Pathways of long-chain alkane sythesis in microbes has been studied. Two enzymes, acyl-ACP reductase(AAR) and aldehyde decarbonylase(ADC), were heterologously expressed in E.coli to reduce fatty acyl-ACPs to corresponding aldehydes and then convert them to alkanes[3]. Fatty aldehydes can also be produced from fatty acids and fatty acyl-CoAs, catalyzed by acyl-CoA reductases(ACR) and carboxylic acid reductase(CAR) respectively, which have been identified in many species[4,5].</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p> Pathways of long-chain alkane sythesis in microbes has been studied. Two enzymes, acyl-ACP reductase(AAR) and aldehyde decarbonylase(ADC), were heterologously expressed in E.coli to reduce fatty acyl-ACPs to corresponding aldehydes and then convert them to alkanes[3]. Fatty aldehydes can also be produced from fatty acids and fatty acyl-CoAs, catalyzed by acyl-CoA reductases(ACR) and carboxylic acid reductase(CAR) respectively, which have been identified in many species[4,5].</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Figure 2.</b>&nbsp; Typical alkane biosynthesis pathways </div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b>Figure 2.</b>&nbsp; Typical alkane biosynthesis pathways </div></div></td></tr>
</table>XIAOKEhttp://2013.igem.org/wiki/index.php?title=Team:Tianjin/Project/Background&diff=324597&oldid=prevXIAOKE at 15:16, 22 October 20132013-10-22T15:16:34Z<p></p>
<a href="http://2013.igem.org/wiki/index.php?title=Team:Tianjin/Project/Background&diff=324597&oldid=320177">Show changes</a>XIAOKEhttp://2013.igem.org/wiki/index.php?title=Team:Tianjin/Project/Background&diff=320177&oldid=prevXIAOKE at 09:52, 17 October 20132013-10-17T09:52:13Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> if(s > t - 100){</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> if(s > t - 100){</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> $('.fixed').css('position','fixed');</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> $('.fixed').css('position','fixed');</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> if(s + fh - <del class="diffchange diffchange-inline">2000 </del>> mh){</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> if(s + fh - <ins class="diffchange diffchange-inline">4000 </ins>> mh){</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> $('.fixed').css('top',mh-s-fh+'px'); </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> $('.fixed').css('top',mh-s-fh+'px'); </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> }</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> }</div></td></tr>
</table>XIAOKEhttp://2013.igem.org/wiki/index.php?title=Team:Tianjin/Project/Background&diff=320173&oldid=prevXIAOKE at 09:48, 17 October 20132013-10-17T09:48:10Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 09:48, 17 October 2013</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment/Endo-Induce">In vivo <del class="diffchange diffchange-inline">alkane sensing test</del></a> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment/Endo-Induce"<ins class="diffchange diffchange-inline">><i</ins>> In vivo <ins class="diffchange diffchange-inline"></i>Alkane Sensing Test</ins></a> </div></td></tr>
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</table>XIAOKE