From 2013.igem.org
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| - | <h7><font color=black><p style = "text-align:center; font-size:35px;"><b>STRAINS TESTED</b></p><br/>
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| - | <p style = "font-size:18px;"><b>BW25113 WT</b><br/>
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| - | <b>MG1655 WT</b>
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| - | <b>BW25113 pEBS-csgD</b><br/>
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| - | <b>BW25113 pEBS-csgD</b><br/>The protein CsgD is a regulator of genes involved in curli assembly, induced in mid-exponential phase that are active in stationary phase. It positively controls σSexpression; its deletion should in principle interfere with biofilm formation. [1] <br/>
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| - | <b>BW25113 pEBS-fimB</b><br/>The gene fimB mediates off to on switching of fim operon, while fimE mediates on to off switching of the fim operon. Its deletion should in principle repress the fim operon, while its overexpression should in principle encourage fimbriae formation. [2]<br/>
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| - | <b>BW25113 pEBS-mlrA</b><br/>MlrA stands for a merR-like regulator A, which regulates curli production. Its overexpression should in principle suppress curli formation, while its deletion should in principle encourage curli formation. [3]</br>
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| - | <b>BW25113 pEBS-ompA </b><br/>The protein OmpA influences cellulose production by
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| - | repressing cellulose production with CpxRA stress response system; it is
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| - | overexpressed in biofilm formation and repressed when cells are exposed to
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| - | visible light. [4]</br>
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Latest revision as of 00:42, 28 September 2013
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