Team:Toronto/Project/Elements

STRAINS TESTED

BW25113 WT
MG1655 WT BW25113 pEBS-csgD
BW25113 pEBS-csgD
The protein CsgD is a regulator of genes involved in curli assembly, induced in mid-exponential phase that are active in stationary phase. It positively controls σSexpression; its deletion should in principle interfere with biofilm formation. [1]
BW25113 pEBS-fimB
The gene fimB mediates off to on switching of fim operon, while fimE mediates on to off switching of the fim operon. Its deletion should in principle repress the fim operon, while its overexpression should in principle encourage fimbriae formation. [2]
BW25113 pEBS-mlrA
MlrA stands for a merR-like regulator A, which regulates curli production. Its overexpression should in principle suppress curli formation, while its deletion should in principle encourage curli formation. [3]
BW25113 pEBS-ompA
The protein OmpA influences cellulose production by repressing cellulose production with CpxRA stress response system; it is overexpressed in biofilm formation and repressed when cells are exposed to visible light. [4]