Team:Tuebingen/Notebook/Protocols

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General and E. coli protocols

• SOB medium
• LB medium
• TAE buffer
• LB plates
• DNA-ladder-buffer mix
• Gelelectrophoresis
• Inoue buffer
• Chemo-competent E. coli cells
• E. coli transformation
• Ligation of digested parts and vectors
• Glycerol stocks
• PCR
• 3A-Assembly
• Preparative restriction digest
• Control restriction digest
• Plasmid Preparation
• Gel Extraction
• Colony-PCR

Saccharomyces cerevisiae-specific protocols

• YPD medium
• SC DropOut
• ONE-STEP buffer
• Low Iron Medium (LIM)
• Yeast transformation
• Sample preparation and fluorescence measurement using the Tecan Reader