Team:Tuebingen/Notebook/Protocols/glycerol-stocks

From 2013.igem.org

(Difference between revisions)
(Created page with "{{:Team:Tuebingen/Template/Tuebingen}} <html> <head> <style> </style> </head> <body> <div id="actualHeadingOne"> Glycerol Stocks </div> <div id="actualContent"> <a href="h...")
Line 16: Line 16:
<div id="actualContent">  
<div id="actualContent">  
-
<a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 16px">Back to Protocols</a>
+
<a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a>
 +
<p>&nbsp;</p>
 +
 +
<h3>Procedure</h3>
 +
 +
<ol>
 +
  <li>Inoculate 5 mL <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/plates">LB-medium</a> with one single colony from a fresh plate (e.g. after <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/trafo">transformation</a>) and incubate at 37 °C and 220 rpm over night.</li>
 +
  <li>On the next day, put 0.5 mL autoclaved glycerol (50 %) in an autoclaved 2 mL Eppendorf-tube.</li>
 +
  <li>Add 1 mL of cell-culture and mix.</li>
 +
</ol>
</div>
</div>

Revision as of 00:31, 4 October 2013

Return to iGEM Main Page.

Glycerol Stocks
Back to Protocols

 

Procedure

  1. Inoculate 5 mL LB-medium with one single colony from a fresh plate (e.g. after transformation) and incubate at 37 °C and 220 rpm over night.
  2. On the next day, put 0.5 mL autoclaved glycerol (50 %) in an autoclaved 2 mL Eppendorf-tube.
  3. Add 1 mL of cell-culture and mix.