Team:Tuebingen/Notebook/Protocols/ladder-mix

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Revision as of 23:01, 3 October 2013

DNA-Ladder Mix

Procedure

Dilute 6x loading buffer with Aqua dest. 1:6 (e.g. 2 µL buffer + 10 µL Aqua dest. - yields 12 µL 1x loading buffer). Mix 1.5 µL DNA-ladder with 8.5 µL 1x loading buffer - yields 10 µL ladder-buffer-mix.

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 <div id="actualContent">
 <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a>
-<p>&nbsp;</p>
-<h3>For 1 pocket:</h3>
-<h3>Reagents</h3>
-<table border="0">
-   <colgroup>
-    <col width="80">
-    <col width="500">
-  </colgroup>
-  <tr>
-    <td style="text-align: center">1.0 g</td>
-    <td>Agarose</td>
-  </tr>
-  <tr>
-    <td style="text-align: center">100 mL</td>
-    <td>1x TAE (dilute <a href="/Team:Tuebingen/Notebook/Protocols/tae">50x TAE</a> accordingly)</td>
-  </tr>
-  <tr>
-    <td style="text-align: center">1.7 µL</td>
-    <td><a href="http://en.wikipedia.org/wiki/GelRed">GelRed</a></td>
-  </tr>
-  <tr>
-    <td style="text-align: center">1 µL</td>
-    <td>1 x loading buffer (dilute with Aqua dest. if necessary) per gel-pocket</td>
-  </tr>
-  <tr>
-    <td style="text-align: center">10 µL</td>
-    <td>DNA-ladder</td>
-  </tr>
-</table>
 <p>&nbsp;</p>
 <h3>Procedure</h3>
-<p>Add agarose to TAE buffer and cook in microwave until completely dissolved. Add GelRed to the liquid (cooling down is not necessary). Prepare casting of gel and let liquid cool down a bit. Cast gel and let solidify.</p>
+<p>Dilute 6x loading buffer with Aqua dest. 1:6 (e.g. 2 µL buffer + 10 µL Aqua dest. - yields 12 µL 1x loading buffer). Mix 1.5 µL DNA-ladder with 8.5 µL 1x loading buffer - yields 10 µL ladder-buffer-mix.</p>
 <p>