Team:Tuebingen/Notebook/Protocols/ladder-mix

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DNA-Ladder Mix
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DNA-Ladder-Buffer Mix
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<a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a>
 
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<p>&nbsp;</p>
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<h3>Procedure</h3>
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<p>Dilute 6x loading buffer with Aqua dest. 1:6 (e.g. 2 µL 6x loading buffer + 10 µL Aqua dest. - yields 12 µL 1x loading buffer). Mix 1.5 µL DNA-ladder (e.g. <a href="https://www.neb.com/products/n3200-2-log-dna-ladder-01-100-kb">NEB 2-Log DNA Ladder</a>) with 8.5 µL 1x loading buffer. <br> Procedure yields 10 µL ladder-buffer mix.</p>
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<h3>For 1 pocket:</h3>
 
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<h3>Reagents</h3>
 
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<table border="0">
 
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  <colgroup>
 
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    <col width="80">
 
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    <col width="500">
 
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    <td style="text-align: center">1.0 g</td>
 
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    <td>Agarose</td>
 
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    <td style="text-align: center">100 mL</td>
 
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    <td>1x TAE (dilute <a href="/Team:Tuebingen/Notebook/Protocols/tae">50x TAE</a> accordingly)</td>
 
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    <td style="text-align: center">1.7 µL</td>
 
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    <td><a href="http://en.wikipedia.org/wiki/GelRed">GelRed</a></td>
 
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    <td style="text-align: center">1 µL</td>
 
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    <td>1 x loading buffer (dilute with Aqua dest. if necessary) per gel-pocket</td>
 
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    <td style="text-align: center">10 µL</td>
 
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    <td>DNA-ladder</td>
 
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</table>
 
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<p>&nbsp;</p>
 
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<h3>Procedure</h3>
 
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<p>Add agarose to TAE buffer and cook in microwave until completely dissolved. Add GelRed to the liquid (cooling down is not necessary). Prepare casting of gel and let liquid cool down a bit. Cast gel and let solidify.</p>
 
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<a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a>
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Latest revision as of 12:08, 4 October 2013

Return to iGEM Main Page.

DNA-Ladder-Buffer Mix

Procedure

Dilute 6x loading buffer with Aqua dest. 1:6 (e.g. 2 µL 6x loading buffer + 10 µL Aqua dest. - yields 12 µL 1x loading buffer). Mix 1.5 µL DNA-ladder (e.g. NEB 2-Log DNA Ladder) with 8.5 µL 1x loading buffer.
Procedure yields 10 µL ladder-buffer mix.

Back to Protocols


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