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Team:Tuebingen/Notebook/Protocols/ladder-mix
From 2013.igem.org
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<p>Dilute 6x loading buffer with Aqua dest. 1:6 (e.g. 2 µL 6x loading buffer + 10 µL Aqua dest. - yields 12 µL 1x loading buffer). Mix 1.5 µL DNA-ladder (e.g. <a href="https://www.neb.com/products/n3200-2-log-dna-ladder-01-100-kb">NEB 2-Log DNA Ladder</a>) with 8.5 µL 1x loading buffer. <br> Procedure yields 10 µL ladder-buffer mix.</p> | <p>Dilute 6x loading buffer with Aqua dest. 1:6 (e.g. 2 µL 6x loading buffer + 10 µL Aqua dest. - yields 12 µL 1x loading buffer). Mix 1.5 µL DNA-ladder (e.g. <a href="https://www.neb.com/products/n3200-2-log-dna-ladder-01-100-kb">NEB 2-Log DNA Ladder</a>) with 8.5 µL 1x loading buffer. <br> Procedure yields 10 µL ladder-buffer mix.</p> | ||
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<a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | ||
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Latest revision as of 12:08, 4 October 2013
DNA-Ladder-Buffer Mix
Procedure
Dilute 6x loading buffer with Aqua dest. 1:6 (e.g. 2 µL 6x loading buffer + 10 µL Aqua dest. - yields 12 µL 1x loading buffer). Mix 1.5 µL DNA-ladder (e.g. NEB 2-Log DNA Ladder) with 8.5 µL 1x loading buffer.
Procedure yields 10 µL ladder-buffer mix.
