Team:Tuebingen/Notebook/Protocols/plates

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<a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 16px">Back to Protocols</a>
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<a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a>
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<p>&nbsp;</p>
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</div>
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<h3>Reagents</h3>
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<table border="0">
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  <colgroup>
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    <col width="80">
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    <col width="300">
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  </colgroup>
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  <tr>
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    <td style="text-align: center">16.0 g</td>
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    <td>Agar</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">1 L</td>
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    <td><a href="/Team:Tuebingen/Notebook/Protocols/lb">LB medium</a></td>
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  </tr>
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  <tr>
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    <td style="text-align: center">100 mL</td>
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    <td>EDTA (c = 0.5 M ; pH 8.0)</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">10 mL</td>
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    <td>KCl (c = 250 mM)</td>
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  </tr>
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</table>
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<p>&nbsp;</p>
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<h3>Procedure</h3>
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<p>Dissolve all reagents in 1000 mL Aqua bidest. Result: <b>50x concentration</b>.</p>
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Revision as of 22:08, 3 October 2013

Return to iGEM Main Page.

LB Plates
Back to Protocols

 

Reagents

16.0 g Agar
1 L LB medium
100 mL EDTA (c = 0.5 M ; pH 8.0)
10 mL KCl (c = 250 mM)

 

Procedure

Dissolve all reagents in 1000 mL Aqua bidest. Result: 50x concentration.