Team:Tuebingen/Notebook/Protocols/plates

From 2013.igem.org

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<h3>Procedure</h3>
<h3>Procedure</h3>
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<p>Add agar to LB medium and heat in microwave until all agar has completely dissolved. <u>Perform next steps at laminar flow cabinet!</u> Cast plates in petri dishes (approx. 20 mL per plate).</p>
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<p>Add agar to LB medium and heat in microwave until all agar has completely dissolved. Cover flask with lid. <u>Perform next step at laminar flow cabinet!</u> Let cool down a bit. If required, add antibiotics to Agar-LB medium. Cast plates in petri dishes (approx. 20 mL per plate).</p>
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Revision as of 22:17, 3 October 2013

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LB Plates
Back to Protocols

 

Reagents

16.0 g Agar
1000 mL LB medium

 

Procedure

Add agar to LB medium and heat in microwave until all agar has completely dissolved. Cover flask with lid. Perform next step at laminar flow cabinet! Let cool down a bit. If required, add antibiotics to Agar-LB medium. Cast plates in petri dishes (approx. 20 mL per plate).