"
Team:Tuebingen/Notebook/Protocols/plates
From 2013.igem.org
(Difference between revisions)
| (5 intermediate revisions not shown) | |||
| Line 16: | Line 16: | ||
<div id="actualContent"> | <div id="actualContent"> | ||
| - | |||
| - | |||
| - | |||
<h3>Reagents</h3> | <h3>Reagents</h3> | ||
| Line 33: | Line 30: | ||
<tr> | <tr> | ||
| - | <td style="text-align: center"> | + | <td style="text-align: center">1000 mL</td> |
<td><a href="/Team:Tuebingen/Notebook/Protocols/lb">LB medium</a></td> | <td><a href="/Team:Tuebingen/Notebook/Protocols/lb">LB medium</a></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td style="text-align: center"> | + | <td style="text-align: center"></td> |
| - | <td> | + | <td>Antibiotics</td> |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
</tr> | </tr> | ||
</table> | </table> | ||
| Line 51: | Line 43: | ||
<h3>Procedure</h3> | <h3>Procedure</h3> | ||
| - | <p> | + | <p>Add agar to LB medium and heat in microwave until all agar has completely dissolved. Cover flask with lid. <u>Perform next step at laminar flow cabinet!</u> Let cool down a bit. If required, add antibiotics to Agar-LB medium. Cast plates in petri dishes (approx. 20 mL per plate). Store plates at 4 °C.</p> |
| + | |||
| + | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | ||
</div> | </div> | ||
<!-- FOOTER --- IMPORTANT FOR NAVMENU-LENGTH!!! --> | <!-- FOOTER --- IMPORTANT FOR NAVMENU-LENGTH!!! --> | ||
Latest revision as of 12:08, 4 October 2013
LB Plates
Reagents
| 16.0 g | Agar |
| 1000 mL | LB medium |
| Antibiotics |
Procedure
Add agar to LB medium and heat in microwave until all agar has completely dissolved. Cover flask with lid. Perform next step at laminar flow cabinet! Let cool down a bit. If required, add antibiotics to Agar-LB medium. Cast plates in petri dishes (approx. 20 mL per plate). Store plates at 4 °C.
Back to Protocols
