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Team:Tuebingen/Notebook/Protocols/tae
From 2013.igem.org
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| - | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: | + | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> |
| + | <p> </p> | ||
| - | </ | + | <h3>Reagents</h3> |
| + | <table border="0"> | ||
| + | <colgroup> | ||
| + | <col width="80"> | ||
| + | <col width="300"> | ||
| + | </colgroup> | ||
| + | <tr> | ||
| + | <td style="text-align: center">242.0 g</td> | ||
| + | <td>Tris Base</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">57.1 mL</td> | ||
| + | <td>Glacial acetic acidtd> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">100 mL</td> | ||
| + | <td>EDTA (c = 0.5 M ; pH 8.0)</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center">10 mL</td> | ||
| + | <td>KCl (c = 250 mM)</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | <p> </p> | ||
| + | |||
| + | <h3>Procedure</h3> | ||
| + | <p>Dissolve all reagents in 1000 mL Aqua bidest. Result: <b>50x concentration</b>.</p> | ||
| + | |||
| + | </div> | ||
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Revision as of 22:05, 3 October 2013
TAE Buffer
Back to Protocols
Reagents
| 242.0 g | Tris Base |
| 57.1 mL | Glacial acetic acidtd> |
| 100 mL | EDTA (c = 0.5 M ; pH 8.0) |
| 10 mL | KCl (c = 250 mM) |
Procedure
Dissolve all reagents in 1000 mL Aqua bidest. Result: 50x concentration.
