Team:Tuebingen/Notebook/Protocols/tae

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<a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 16px">Back to Protocols</a>
 
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<h3>Reagents</h3>
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    <td style="text-align: center">242.0 g</td>
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    <td>Tris Base</td>
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    <td style="text-align: center">57.1 mL</td>
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    <td>Glacial acetic acid</td>
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    <td style="text-align: center">100 mL</td>
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    <td>EDTA (c = 0.5 M ; pH 8.0)</td>
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    <td style="text-align: center">10 mL</td>
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    <td>KCl (c = 250 mM)</td>
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<p>&nbsp;</p>
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<h3>Procedure</h3>
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<p>Dissolve all reagents in 1000 mL Aqua bidest. Store at room temperature.
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<br>Procedure yields <b>50x concentration</b>.</p>
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<a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a>
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Latest revision as of 12:08, 4 October 2013

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TAE Buffer

Reagents

242.0 g Tris Base
57.1 mL Glacial acetic acid
100 mL EDTA (c = 0.5 M ; pH 8.0)
10 mL KCl (c = 250 mM)

 

Procedure

Dissolve all reagents in 1000 mL Aqua bidest. Store at room temperature.
Procedure yields 50x concentration.

Back to Protocols