Team:Tuebingen/Notebook/Protocols/tecanreader

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<p>The Tecan reader is used for flourescence spectroscopy. Thus, tested cells should express some sort of flourescent protein. We mainly tested yeast cells that express mOrange - <a href="http://parts.igem.org/Part:BBa_E2050">BBa_E2050</a>.</p>
<h3>Procedure</h3>
<h3>Procedure</h3>
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  <li>Inoculate 5 mL YPD medium with yeast cells. Create 1:10 and 1:100 dilutions of this liquid culture. Store all at 30°C.</li>
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Revision as of 19:55, 4 October 2013

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Tecan Reader

The Tecan reader is used for flourescence spectroscopy. Thus, tested cells should express some sort of flourescent protein. We mainly tested yeast cells that express mOrange - BBa_E2050.

Procedure

  1. Inoculate 5 mL YPD medium with yeast cells. Create 1:10 and 1:100 dilutions of this liquid culture. Store all at 30°C.

 

Back to Protocols