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Team:Tuebingen/Notebook/Protocols/trafo
From 2013.igem.org
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<li>Add 0.9 mL of <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/lb">LB-medium</a> and let bacteria grow at 37°C.</li> | <li>Add 0.9 mL of <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/lb">LB-medium</a> and let bacteria grow at 37°C.</li> | ||
<li>Plate on <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/plates">LB plates</a> + required amount of antibiotic and incubate at 37 °C over night.</li> | <li>Plate on <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/plates">LB plates</a> + required amount of antibiotic and incubate at 37 °C over night.</li> | ||
| + | <li>If necessary, store plates at 4 °C.</li> | ||
</ol> | </ol> | ||
Revision as of 02:58, 4 October 2013
E. coli Transformation
Back to Protocols
Procedure
- Add desired volume of (plasmid) DNA (not more than 1/10 vol.) to 100 µL E. coli culture (i.e. to aliquot) in Eppendorf-tube.
- Incubate on ice for 30 min.
- Heat shock at 42 °C for 90 s.
- Incubate 10 min on ice.
- Add 0.9 mL of LB-medium and let bacteria grow at 37°C.
- Plate on LB plates + required amount of antibiotic and incubate at 37 °C over night.
- If necessary, store plates at 4 °C.
