Team:Tuebingen/Notebook/Protocols/trafo

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<h3>Procedure</h3>
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Latest revision as of 12:12, 4 October 2013

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E. coli Transformation

Procedure

  1. Add desired volume of (plasmid) DNA (not more than 1/10 vol.) to 100 µL E. coli culture (i.e. to aliquot) in Eppendorf-tube.
  2. Incubate on ice for 30 min.
  3. Heat shock at 42 °C for 90 s.
  4. Incubate 10 min on ice.
  5. Add 0.9 mL of LB-medium and let bacteria grow at 37°C.
  6. Plate on LB plates + required amount of antibiotic and incubate at 37 °C over night.
  7. If necessary, store plates at 4 °C.

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