http://2013.igem.org/wiki/index.php?title=Team:Tuebingen/Notebook/Protocols/trafo&feed=atom&action=history
Team:Tuebingen/Notebook/Protocols/trafo - Revision history
2024-03-28T23:21:07Z
Revision history for this page on the wiki
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http://2013.igem.org/wiki/index.php?title=Team:Tuebingen/Notebook/Protocols/trafo&diff=291241&oldid=prev
SvenB at 12:12, 4 October 2013
2013-10-04T12:12:21Z
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SvenB
http://2013.igem.org/wiki/index.php?title=Team:Tuebingen/Notebook/Protocols/trafo&diff=291193&oldid=prev
SvenB at 12:09, 4 October 2013
2013-10-04T12:09:35Z
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SvenB
http://2013.igem.org/wiki/index.php?title=Team:Tuebingen/Notebook/Protocols/trafo&diff=291018&oldid=prev
SvenB at 12:03, 4 October 2013
2013-10-04T12:03:36Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Procedure</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Procedure</h3></div></td></tr>
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SvenB
http://2013.igem.org/wiki/index.php?title=Team:Tuebingen/Notebook/Protocols/trafo&diff=287576&oldid=prev
SvenB at 02:58, 4 October 2013
2013-10-04T02:58:03Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Add 0.9 mL of <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/lb">LB-medium</a> and let bacteria grow at 37°C.</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Add 0.9 mL of <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/lb">LB-medium</a> and let bacteria grow at 37°C.</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Plate on <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/plates">LB plates</a> + required amount of antibiotic and incubate at 37 °C over night.</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li>Plate on <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/plates">LB plates</a> + required amount of antibiotic and incubate at 37 °C over night.</li></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <li>If necessary, store plates at 4 °C.</li></ins></div></td></tr>
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SvenB
http://2013.igem.org/wiki/index.php?title=Team:Tuebingen/Notebook/Protocols/trafo&diff=286194&oldid=prev
SvenB at 00:03, 4 October 2013
2013-10-04T00:03:32Z
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Add desired volume of (plasmid) DNA (not more than 1/10 vol.) to 100 µL <i>E. coli</i> culture (i.e. to <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/chemo-competentcells">aliquot</a>) in Eppendorf-tube. </li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Incubate on ice for 30 min.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Heat shock at 42 °C for 90 s.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Incubate 10 min on ice.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Add 0.9 mL of <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/lb">LB-medium</a> and let bacteria grow at 37°C.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <li>Plate on <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/plates">LB plates</a> + required amount of antibiotic and incubate at 37 °C over night.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"></ol</ins>></div></td></tr>
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SvenB
http://2013.igem.org/wiki/index.php?title=Team:Tuebingen/Notebook/Protocols/trafo&diff=284135&oldid=prev
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2013-10-03T21:29:07Z
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