Team:Tuebingen/Project/Plasmids

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<h3>"Receptor"-Plasmids</h3>
<h3>"Receptor"-Plasmids</h3>
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<img src="https://static.igem.org/mediawiki/2012/d/dc/PRS313_Padh1_Danio_Tadh1.png" title="mPR Dr">
<img src="https://static.igem.org/mediawiki/2012/d/dc/PRS313_Padh1_Danio_Tadh1.png" title="mPR Dr">

Revision as of 16:24, 3 October 2013

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Plasmids

We have designed our system to consist of three plasmids that will be be transformed into S. cerevisiae. Each plasmid will contain one part of our project - receptor, inverter, reporter - whereby there will be two receptor plasmids (one with mPR Dr, one with mPR Xl), two inverter plasmids (one with ROX1, one with MIG1), and two reporter plasmids (one with Psuc2, one with Panb1).

Each plasmid will carry three parts, namely a promoter, a protein coding sequence, and the ADH1 terminator (Tadh1). We have retreived Tadh1 from the registry - BBa_K801012.

"Receptor"-Plasmids