Team:Tuebingen/Project/Plasmids

From 2013.igem.org

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<p>pRS315 containing Pfet3, mig1, and Tadh1:</p>
<p>pRS315 containing Pfet3, mig1, and Tadh1:</p>
<img src="https://static.igem.org/mediawiki/2012/0/0c/PRS315_Pfet3_mig1_Tadh1.png" title="mig1">
<img src="https://static.igem.org/mediawiki/2012/0/0c/PRS315_Pfet3_mig1_Tadh1.png" title="mig1">
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<h3>"Reporter"-Plasmids</h3>
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<p>pRS316 containing Panb1, luciferase (luc), and Tadh1:</p>
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<img src="https://static.igem.org/mediawiki/2012/9/9e/PRS316_Psuc2_Luciferase_Tadh1.png" title="rox1">
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<p>pRS316 containing Psuc2, luciferase (luc), and Tadh1:</p>
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<img src="https://static.igem.org/mediawiki/2012/9/9e/PRS316_Psuc2_Luciferase_Tadh1.png" title="mig1">
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Revision as of 16:38, 3 October 2013

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Plasmids

We have designed our system to consist of three plasmids that will be be transformed into S. cerevisiae. Each plasmid will contain one part of our project - receptor, inverter, reporter - whereby there will be two receptor plasmids (one with mPR Dr, one with mPR Xl), two inverter plasmids (one with ROX1, one with MIG1), and two reporter plasmids (one with Psuc2, one with Panb1).

Each plasmid will carry three parts, namely a promoter, a protein coding sequence, and the ADH1 terminator (Tadh1). We have retreived Tadh1 from the registry - BBa_K801012.

"Receptor"-Plasmids

pRS313 containing Padh1, mPR Dr, and Tadh1:

pRS313 containing Padh1, mPR Xl, and Tadh1:

"Inverter"-Plasmids

pRS315 containing Pfet3, rox1, and Tadh1:

pRS315 containing Pfet3, mig1, and Tadh1:

"Reporter"-Plasmids

pRS316 containing Panb1, luciferase (luc), and Tadh1:

pRS316 containing Psuc2, luciferase (luc), and Tadh1: