http://2013.igem.org/wiki/index.php?title=Team:Tuebingen/Project/Plasmids&feed=atom&action=historyTeam:Tuebingen/Project/Plasmids - Revision history2024-03-28T12:11:04ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:Tuebingen/Project/Plasmids&diff=282971&oldid=prevSvenB at 20:23, 3 October 20132013-10-03T20:23:50Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>References</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>References</h3></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>SIKORSKI, R. S. & HIETER, P. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics, 122, 19-27.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>SIKORSKI, R. S. & HIETER, P. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics, 122, 19-27.</p></div></td></tr>
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</table>SvenBhttp://2013.igem.org/wiki/index.php?title=Team:Tuebingen/Project/Plasmids&diff=279475&oldid=prevSvenB at 17:13, 3 October 20132013-10-03T17:13:25Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We have designed our system to consist of three plasmids that will be be transformed into <i>S. cerevisiae</i>. Each plasmid will contain one part of our project - receptor, inverter, reporter - whereby there will be two receptor plasmids (one with mPR Dr, one with mPR Xl), two inverter plasmids (one with ROX1, one with MIG1), and two reporter plasmids (one with Psuc2, one with Panb1).</p> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We have designed our system to consist of three plasmids that will be be transformed into <i>S. cerevisiae</i>. Each plasmid will contain one part of our project - receptor, inverter, reporter - whereby there will be two receptor plasmids (one with mPR Dr, one with mPR Xl), two inverter plasmids (one with ROX1, one with MIG1), and two reporter plasmids (one with Psuc2, one with Panb1).</p> </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>All our constructs are cloned into the pRS plasmid. The pRS plasmid is derived from the E. coli plasmid <a href=http://en.wikipedia.org/wiki/PBluescript>pBLUESCRIPT</a> (Stratagene, La Jolla, CA, USA) and serves as a shuttle vector between Saccharomyces cerevisiae and bacteria like E. coli (Sikorski and Hieter, 1989). pRS offers built in blue/white screening and is a high copy plasmid in bacteria (Sikorski and Hieter, 1989) thus plasmid extractions from E. coli yield high quantities of plasmid DNA that can be used for yeast transformations.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>All our constructs are cloned into the pRS plasmid. The pRS plasmid is derived from the <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>plasmid <a href=http://en.wikipedia.org/wiki/PBluescript>pBLUESCRIPT</a> (Stratagene, La Jolla, CA, USA) and serves as a shuttle vector between <ins class="diffchange diffchange-inline"><i></ins>Saccharomyces cerevisiae<ins class="diffchange diffchange-inline"></i> </ins>and bacteria like <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>(Sikorski and Hieter, 1989). pRS offers built in blue/white screening and is a high copy plasmid in bacteria (Sikorski and Hieter, 1989) thus plasmid extractions from <ins class="diffchange diffchange-inline"><i></ins>E. coli<ins class="diffchange diffchange-inline"></i> </ins>yield high quantities of plasmid DNA that can be used for yeast transformations.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Each plasmid will carry three parts, namely a promoter, a protein coding sequence, and the ADH1 terminator (Tadh1). We have retreived Tadh1 from the Registry - <a href="http://parts.igem.org/Part:BBa_K801012">BBa_K801012</a>.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Each plasmid will carry three parts, namely a promoter, a protein coding sequence, and the ADH1 terminator (Tadh1). We have retreived Tadh1 from the Registry - <a href="http://parts.igem.org/Part:BBa_K801012">BBa_K801012</a>.</p></div></td></tr>
</table>SvenBhttp://2013.igem.org/wiki/index.php?title=Team:Tuebingen/Project/Plasmids&diff=279401&oldid=prevSvenB at 17:09, 3 October 20132013-10-03T17:09:17Z<p></p>
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</table>SvenBhttp://2013.igem.org/wiki/index.php?title=Team:Tuebingen/Project/Plasmids&diff=279389&oldid=prevSvenB at 17:08, 3 October 20132013-10-03T17:08:43Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>All our constructs are cloned into the pRS plasmid. The pRS plasmid is derived from the E. coli plasmid <a href=http://en.wikipedia.org/wiki/PBluescript>pBLUESCRIPT</a> (Stratagene, La Jolla, CA, USA) and serves as a shuttle vector between Saccharomyces cerevisiae and bacteria like E. coli (Sikorski and Hieter, 1989). pRS offers built in blue/white screening and is a high copy plasmid in bacteria (Sikorski and Hieter, 1989) thus plasmid extractions from E. coli yield high quantities of plasmid DNA that can be used for yeast transformations.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>All our constructs are cloned into the pRS plasmid. The pRS plasmid is derived from the E. coli plasmid <a href=http://en.wikipedia.org/wiki/PBluescript>pBLUESCRIPT</a> (Stratagene, La Jolla, CA, USA) and serves as a shuttle vector between Saccharomyces cerevisiae and bacteria like E. coli (Sikorski and Hieter, 1989). pRS offers built in blue/white screening and is a high copy plasmid in bacteria (Sikorski and Hieter, 1989) thus plasmid extractions from E. coli yield high quantities of plasmid DNA that can be used for yeast transformations.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Each plasmid will carry three parts, namely a promoter, a protein coding sequence, and the ADH1 terminator (Tadh1). We have retreived Tadh1 from the <del class="diffchange diffchange-inline">registry </del>- <a href="http://parts.igem.org/Part:BBa_K801012">BBa_K801012</a>.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Each plasmid will carry three parts, namely a promoter, a protein coding sequence, and the ADH1 terminator (Tadh1). We have retreived Tadh1 from the <ins class="diffchange diffchange-inline">Registry </ins>- <a href="http://parts.igem.org/Part:BBa_K801012">BBa_K801012</a>.</p></div></td></tr>
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</table>SvenBhttp://2013.igem.org/wiki/index.php?title=Team:Tuebingen/Project/Plasmids&diff=279279&oldid=prevSvenB at 17:03, 3 October 20132013-10-03T17:03:25Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We have designed our system to consist of three plasmids that will be be transformed into <i>S. cerevisiae</i>. Each plasmid will contain one part of our project - receptor, inverter, reporter - whereby there will be two receptor plasmids (one with mPR Dr, one with mPR Xl), two inverter plasmids (one with ROX1, one with MIG1), and two reporter plasmids (one with Psuc2, one with Panb1).</p> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We have designed our system to consist of three plasmids that will be be transformed into <i>S. cerevisiae</i>. Each plasmid will contain one part of our project - receptor, inverter, reporter - whereby there will be two receptor plasmids (one with mPR Dr, one with mPR Xl), two inverter plasmids (one with ROX1, one with MIG1), and two reporter plasmids (one with Psuc2, one with Panb1).</p> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The plasmid <del class="diffchange diffchange-inline">we are using </del>is the pRS plasmid.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p>All our constructs are cloned into the pRS plasmid. </ins>The <ins class="diffchange diffchange-inline">pRS </ins>plasmid is <ins class="diffchange diffchange-inline">derived from </ins>the <ins class="diffchange diffchange-inline">E. coli plasmid <a href=http://en.wikipedia.org/wiki/PBluescript>pBLUESCRIPT</a> (Stratagene, La Jolla, CA, USA) and serves as a shuttle vector between Saccharomyces cerevisiae and bacteria like E. coli (Sikorski and Hieter, 1989). </ins>pRS <ins class="diffchange diffchange-inline">offers built in blue/white screening and is a high copy plasmid in bacteria (Sikorski and Hieter, 1989) thus plasmid extractions from E. coli yield high quantities of </ins>plasmid <ins class="diffchange diffchange-inline">DNA that can be used for yeast transformations</ins>.<ins class="diffchange diffchange-inline"></p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Each plasmid will carry three parts, namely a promoter, a protein coding sequence, and the ADH1 terminator (Tadh1). We have retreived Tadh1 from the registry - <a href="http://parts.igem.org/Part:BBa_K801012">BBa_K801012</a>.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Each plasmid will carry three parts, namely a promoter, a protein coding sequence, and the ADH1 terminator (Tadh1). We have retreived Tadh1 from the registry - <a href="http://parts.igem.org/Part:BBa_K801012">BBa_K801012</a>.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="verticallyPlease"></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="verticallyPlease"></div></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><h3>References</h3></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p>SIKORSKI, R. S. & HIETER, P. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics, 122, 19-27.</p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
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</table>SvenB