Team:Tufts

From 2013.igem.org

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<p>Formed in early 2013,  Tufts' iGEM team dove head first into the world of synthetic biology. Though it is currently a  subgroup of the American Institute of Chemical Engineers, Tufts iGEM plans to apply for independent recognition for Fall 2013.</p>
<p>Formed in early 2013,  Tufts' iGEM team dove head first into the world of synthetic biology. Though it is currently a  subgroup of the American Institute of Chemical Engineers, Tufts iGEM plans to apply for independent recognition for Fall 2013.</p>
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<p>This year's project involves the predatory bacteria Bdellovibrio bacteriovorus, fluorescent proteins, and a population of unfortunate bacteria that will fall prey to the B. bacteriovorus.  The goal is to engineer B. bacteriovorus' genome so that the bacterium will produce a blue fluorescent protein  when it enters its predatory phase and is on the hunt for bacteria to feed on. Once B. bacteriovorus finds and consumes its prey, thus entering its digestive phase, it will fluoresce green.</p>
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<p>For our project, we propose to lay the groundwork for synthetic biology in standardized modification of Bdellovibrio Bacteriovorus by providing a set of toolkit plasmids which will make it possible to express a protein of choice during the different life stages of the predatory bacterium. As a proof of concept we will express different fluorescent proteins during the life stages of attack, digestion, and division. Once completed, we will be introducing a “kill-switch” in the form a riboswitch to ensure greater control over the bacterium</p>
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<p>This could enable B. bacteriovorus to be utilized as a safe and thorough bacteriocide, killing harmful bacteria in open wounds or even in the digestive tract without harming non-bacterial cells and dying once there are no more bacteria to hunt.</p>
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Revision as of 06:30, 20 August 2013


Formed in early 2013, Tufts' iGEM team dove head first into the world of synthetic biology. Though it is currently a subgroup of the American Institute of Chemical Engineers, Tufts iGEM plans to apply for independent recognition for Fall 2013.

For our project, we propose to lay the groundwork for synthetic biology in standardized modification of Bdellovibrio Bacteriovorus by providing a set of toolkit plasmids which will make it possible to express a protein of choice during the different life stages of the predatory bacterium. As a proof of concept we will express different fluorescent proteins during the life stages of attack, digestion, and division. Once completed, we will be introducing a “kill-switch” in the form a riboswitch to ensure greater control over the bacterium



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Contents


Tufts iGem (Draft-JK)

About the Team

Main page: Team

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Team Profile

Main page: Official Team Profile

Summary goes here.

Project

Main page: Project

Summary goes here.

Parts submitted to the Registry

Main page: Parts

Summary goes here.

Modeling

Main page: Modeling

Summary goes here.

Notebook

Main page: Notebook

Summary goes here.

Safety

Main page: Safety

Summary goes here.

Attributions

Main page: Attributions

Summary goes here.