Team:UCL/Labbook/Week7

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<p class="body_text"> <a href="https://2013.igem.org/Team:UCL/LabBook/Week1">Week 1</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week2"> Week 2</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week3"> Week 3</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week4"> Week 4</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week5"> Week 5</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week6"> Week 6</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week7"> Week 7</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week8"> Week 8</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week9"> Week 9</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week10"> Week 10</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week11"> Week 11</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week12"> Week 12</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week13"> Week 13</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week14"> Week 14</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week15"> Week 15</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week16"> Week 16</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week17"> Week 17</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week18"> Week 18</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week19"> Week 19</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week20"> Week 20</a>
<p class="body_text"> <a href="https://2013.igem.org/Team:UCL/LabBook/Week1">Week 1</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week2"> Week 2</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week3"> Week 3</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week4"> Week 4</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week5"> Week 5</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week6"> Week 6</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week7"> Week 7</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week8"> Week 8</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week9"> Week 9</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week10"> Week 10</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week11"> Week 11</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week12"> Week 12</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week13"> Week 13</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week14"> Week 14</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week15"> Week 15</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week16"> Week 16</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week17"> Week 17</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week18"> Week 18</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week19"> Week 19</a> | <a href="https://2013.igem.org/Team:UCL/Labbook/Week20"> Week 20</a>
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</div>
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<div class="gap">
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</div>
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<p class="major_title">July</p>
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<div class="gap">
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</div>
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<div class="full_row">
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<p class="minor_title">Week 7</p>
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<p class="body_text">
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<b>Bacterial Lab</b>
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</p>
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<p class="body_text">
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15th July - The team is introduced to the laboratories which will be used during the summer for both bacterial and mammalian experiments.
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</p>
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<p class="body_text">
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16th July - <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 5X M9 salts</a>, <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> minimal agar</a>,  <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 1.4% molten agar solution</a> and <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 0.1M CaCl2/15% glycerol</a> were prepared for the generation of competent cells. Minimal agar plates were poured and <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> streaked</a> with W3110 Escherichia coli cells and left overnight to incubate at 37C.
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</p>
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<p class="body_text">
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17th July - Very little colony growth was observed from W3110 ''E.coli'' streaked plates. Plates were therefore left to incubate for a further 17 hours.
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</p>
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<p class="body_text">
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18th July - Sufficient colony growth allowed for the selection of a single colony from each plate. This was then inoculated in 5ul  <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> LB media</a> + 100ul 1M MgSO4 and left to incu-shake overnight at 37C.
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</p>
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<p class="body_text">
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19th July - Cultures re-suspended in new <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> LB media</a> and 100µl aliquots placed into individual eppendorf tubes for placement at -80C.
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</p>
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</div>
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<div class="full_row">
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<p class="body_text">
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<b>Mammalian Lab</b>
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</p>
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<p class="body_text">
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17th July -  <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> Mammalian cell culture and maintenance</a> training by Mrs. Ludmilla Ruban. Passaged primary MEF (mouse embryonic fibroblast) cells. Passage 3.
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</p>
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<p class="body_text">
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18th July - MEF passage 4
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</p>
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<p class="body_text">
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19th July - MEF passage 5
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</p>
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</p>
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Revision as of 10:37, 3 October 2013

Lab Weeks

July

Week 7

Bacterial Lab

15th July - The team is introduced to the laboratories which will be used during the summer for both bacterial and mammalian experiments.

16th July - 5X M9 salts, minimal agar, 1.4% molten agar solution and 0.1M CaCl2/15% glycerol were prepared for the generation of competent cells. Minimal agar plates were poured and streaked with W3110 Escherichia coli cells and left overnight to incubate at 37C.

17th July - Very little colony growth was observed from W3110 ''E.coli'' streaked plates. Plates were therefore left to incubate for a further 17 hours.

18th July - Sufficient colony growth allowed for the selection of a single colony from each plate. This was then inoculated in 5ul LB media + 100ul 1M MgSO4 and left to incu-shake overnight at 37C.

19th July - Cultures re-suspended in new LB media and 100µl aliquots placed into individual eppendorf tubes for placement at -80C.

Mammalian Lab

17th July - Mammalian cell culture and maintenance training by Mrs. Ludmilla Ruban. Passaged primary MEF (mouse embryonic fibroblast) cells. Passage 3.

18th July - MEF passage 4

19th July - MEF passage 5