Team:UCL/Labbook/Week7

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22nd July - MEF passage 6. Training ends.
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Sunday 22nd July - MEF passage 6. Training ends.
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Revision as of 13:36, 3 October 2013

Lab Weeks

July

Week 7

Bacterial Lab

Monday 15th July - The team is introduced to the laboratories which will be used during the summer for both bacterial and mammalian experiments.

Tuesday 16th July - 5X M9 salts, minimal agar, 1.4% molten agar solution and 0.1M CaCl2/15% glycerol were prepared for the generation of competent cells. Minimal agar plates were poured and streaked with W3110 Escherichia coli cells and left overnight to incubate at 37C.

Wednesday 17th July - Very little colony growth was observed from W3110 ''E.coli'' streaked plates. Plates were therefore left to incubate for a further 17 hours.

Thursday 18th July - Sufficient colony growth allowed for the selection of a single colony from each plate. This was then inoculated in 5ul LB media + 100ul 1M MgSO4 and left to incu-shake overnight at 37C.

Friday 19th July - Cultures re-suspended in new LB media and 100µl aliquots placed into individual eppendorf tubes for placement at -80C.

Sunday 22nd July - Transformation of our competent cells with plasmid YB3110 was carried out.

Mammalian Lab

Wednesday 17th July - Mammalian cell culture and maintenance training by Mrs. Ludmilla Ruban. Passaged primary MEF (mouse embryonic fibroblast) cells. Passage 3.

Thursday 18th July - MEF passage 4

Friday 19th July - MEF passage 5

Sunday 22nd July - MEF passage 6. Training ends.