"
Team:UCL/Labbook/Week7
From 2013.igem.org
| Line 75: | Line 75: | ||
<p class="body_text"> | <p class="body_text"> | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
The team is introduced to the laboratories which will be used during the summer for both bacterial and mammalian experiments. | The team is introduced to the laboratories which will be used during the summer for both bacterial and mammalian experiments. | ||
</p> | </p> | ||
| Line 81: | Line 82: | ||
<p class="body_text"> | <p class="body_text"> | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
<a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 5X M9 salts</a>, <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> minimal agar</a>, <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 1.4% molten agar solution</a> and <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 0.1M CaCl2/15% glycerol</a> were prepared for the generation of competent cells. Minimal agar plates were poured and <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> streaked</a> with W3110 Escherichia coli cells and left overnight to incubate at 37C. | <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 5X M9 salts</a>, <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> minimal agar</a>, <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 1.4% molten agar solution</a> and <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> 0.1M CaCl2/15% glycerol</a> were prepared for the generation of competent cells. Minimal agar plates were poured and <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> streaked</a> with W3110 Escherichia coli cells and left overnight to incubate at 37C. | ||
</p> | </p> | ||
| Line 87: | Line 89: | ||
<p class="body_text"> | <p class="body_text"> | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
Very little colony growth was observed from W3110 ''E.coli'' streaked plates. Plates were therefore left to incubate for a further 17 hours. | Very little colony growth was observed from W3110 ''E.coli'' streaked plates. Plates were therefore left to incubate for a further 17 hours. | ||
</p> | </p> | ||
| Line 93: | Line 96: | ||
<p class="body_text"> | <p class="body_text"> | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
Sufficient colony growth allowed for the selection of a single colony from each plate. This was then inoculated in 5ul <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> LB media</a> + 100ul 1M MgSO4 and left to incu-shake overnight at 37C. | Sufficient colony growth allowed for the selection of a single colony from each plate. This was then inoculated in 5ul <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> LB media</a> + 100ul 1M MgSO4 and left to incu-shake overnight at 37C. | ||
</p> | </p> | ||
| Line 99: | Line 103: | ||
<p class="body_text"> | <p class="body_text"> | ||
</p> | </p> | ||
| + | <p class="body_text"> | ||
Cultures re-suspended in new <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> LB media</a> and 100µl aliquots placed into individual eppendorf tubes for placement at -80C. | Cultures re-suspended in new <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> LB media</a> and 100µl aliquots placed into individual eppendorf tubes for placement at -80C. | ||
</p> | </p> | ||
Revision as of 23:44, 4 October 2013
Lab Weeks
Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18
July
Week 7
Bacterial Lab
Monday 15th July
The team is introduced to the laboratories which will be used during the summer for both bacterial and mammalian experiments.
Tuesday 16th July
5X M9 salts, minimal agar, 1.4% molten agar solution and 0.1M CaCl2/15% glycerol were prepared for the generation of competent cells. Minimal agar plates were poured and streaked with W3110 Escherichia coli cells and left overnight to incubate at 37C.
Wednesday 17th July
Very little colony growth was observed from W3110 ''E.coli'' streaked plates. Plates were therefore left to incubate for a further 17 hours.
Thursday 18th July
Sufficient colony growth allowed for the selection of a single colony from each plate. This was then inoculated in 5ul LB media + 100ul 1M MgSO4 and left to incu-shake overnight at 37C.
Friday 19th July
Cultures re-suspended in new LB media and 100µl aliquots placed into individual eppendorf tubes for placement at -80C.
Mammalian Lab
Wednesday 17th July
Mammalian cell culture and maintenance training by Mrs. Ludmilla Ruban. Passaged primary MEF (mouse embryonic fibroblast) cells. Passage 3.
Thursday 18th July
MEF passage 4
Friday 19th July
MEF passage 5
