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Team:UCL/Labbook/Week8
From 2013.igem.org
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| + | Sunday 22nd July -<a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> Transformation</a> of our <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> competent cells</a> with plasmid YB3110 was carried out. | ||
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| + | <b>Mammalian Lab</b> | ||
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| + | Sunday 22nd July - MEF passage 6. Training ends. | ||
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Revision as of 13:39, 3 October 2013
Lab Weeks
Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16
Week 8
Bacterial Lab
23rd July - No colony growth was observed on Ampicillin plates indicating no plasmid uptake. transformation was repeated with YB3110.
| Vial | Ampicillin Plate | Plasmid Insertion | Colony Count |
|---|---|---|---|
| 1 (Main) | Yes | Yes | 0 |
| 2 (Positive Control) | No | Yes | 100+ |
| 3 (Negative Control) | Yes | No | 0 |
24th July - No colony growth from main experiment indicating no plasmid uptake. Transformation was repeated.
| Vial | Ampicillin Plate | Plasmid Insertion | Colony Count |
|---|---|---|---|
| 1 (Main) | Yes | Yes | 0 |
| 2 (Positive Control) | No | Yes | 100+ |
| 3 (Negative Control) | Yes | No | 0 |
25th July - No colony growth and hence no plasmid uptake. Transformation was repeated.
| Vial | Ampicillin Plate | Plasmid Insertion | Colony Count |
|---|---|---|---|
| 1 (Main) | Yes | Yes | 0 |
| 2 (Positive Control) | No | Yes | 100+ |
| 3 (Negative Control) | Yes | No | 0 |
26th July - Once again our cells were unsuccessful in taking up the YB3110 plasmid. We concluded that our batch of competent cells were not competent and so these will not be used in any further experiments. A new batch of competent cells are to be generated.
| Vial | Ampicillin Plate | Plasmid Insertion | Colony Count |
|---|---|---|---|
| 1 (Main) | Yes | Yes | 0 |
| 2 (Positive Control) | No | Yes | 100+ |
| 3 (Negative Control) | Yes | No | 0 |
Sunday 22nd July - Transformation of our competent cells with plasmid YB3110 was carried out.
Mammalian Lab
Sunday 22nd July - MEF passage 6. Training ends.
