Team:UCL/Labbook/Week8

From 2013.igem.org

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Monday 22nd July -<a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> Transformation</a> of our <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> competent cells</a> with plasmid YB3110 was carried out.  
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<b><u>Monday 22nd July</u></b> -<a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> Transformation</a> of our <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> competent cells</a> with plasmid YB3110 was carried out.  
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Tuesday 23rd July - No colony growth was observed on  <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> Ampicillin plates</a> indicating no plasmid uptake. <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> transformation</a> was repeated with YB3110.
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<b><u>Tuesday 23rd July</u></b> - No colony growth was observed on  <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> Ampicillin plates</a> indicating no plasmid uptake. <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> transformation</a> was repeated with YB3110.
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Wednesday 24th July - No colony growth from main experiment indicating no plasmid uptake. <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> Transformation</a> was repeated.  
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<b><u>Wednesday 24th July</u></b> - No colony growth from main experiment indicating no plasmid uptake. <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> Transformation</a> was repeated.  
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Thursday 25th July - No colony growth and hence no plasmid uptake. <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> Transformation</a> was repeated.
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<b><u>Thursday 25th July</u></b> - No colony growth and hence no plasmid uptake. <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> Transformation</a> was repeated.
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Friday 26th July - Once again our cells were unsuccessful in taking up the YB3110 plasmid. We concluded that our batch of competent cells were not competent and so these will not be used in any further experiments. A new batch of <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> competent cells</a> are to be generated.  
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<b><u>Friday 26th July</u></b> - Once again our cells were unsuccessful in taking up the YB3110 plasmid. We concluded that our batch of competent cells were not competent and so these will not be used in any further experiments. A new batch of <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="_blank"> competent cells</a> are to be generated.  
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Monday 22nd July - MEF passage 6. Training ends.
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<b><u>Monday 22nd July</u></b> - MEF passage 6. Training ends.
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Revision as of 14:07, 3 October 2013

Lab Weeks

Week 8

Bacterial Lab

Monday 22nd July - Transformation of our competent cells with plasmid YB3110 was carried out.

Tuesday 23rd July - No colony growth was observed on Ampicillin plates indicating no plasmid uptake. transformation was repeated with YB3110.

Vial Ampicillin Plate Plasmid Insertion Colony Count
1 (Main) Yes Yes 0
2 (Positive Control) No Yes 100+
3 (Negative Control) Yes No 0

Wednesday 24th July - No colony growth from main experiment indicating no plasmid uptake. Transformation was repeated.

Vial Ampicillin Plate Plasmid Insertion Colony Count
1 (Main) Yes Yes 0
2 (Positive Control) No Yes 100+
3 (Negative Control) Yes No 0

Thursday 25th July - No colony growth and hence no plasmid uptake. Transformation was repeated.

Vial Ampicillin Plate Plasmid Insertion Colony Count
1 (Main) Yes Yes 0
2 (Positive Control) No Yes 100+
3 (Negative Control) Yes No 0

Friday 26th July - Once again our cells were unsuccessful in taking up the YB3110 plasmid. We concluded that our batch of competent cells were not competent and so these will not be used in any further experiments. A new batch of competent cells are to be generated.

Vial Ampicillin Plate Plasmid Insertion Colony Count
1 (Main) Yes Yes 0
2 (Positive Control) No Yes 100+
3 (Negative Control) Yes No 0

Mammalian Lab

Monday 22nd July - MEF passage 6. Training ends.