Team:UCL/Labbook/Week8

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Lab Weeks

Week 8

Bacterial Lab

Monday 22nd July

Transformation of our competent cells with plasmid YB3110 was carried out.

Tuesday 23rd July

No colony growth was observed on Ampicillin plates indicating no plasmid uptake. Transformation was repeated with YB3110.

Vial Ampicillin Plate Plasmid Insertion Colony Count
1 (Main) Yes Yes 0
2 (Positive Control) No Yes 100+
3 (Negative Control) Yes No 0

Wednesday 24th July

No colony growth from main experiment indicating no plasmid uptake. Transformation was repeated.

Vial Ampicillin Plate Plasmid Insertion Colony Count
1 (Main) Yes Yes 0
2 (Positive Control) No Yes 100+
3 (Negative Control) Yes No 0

Thursday 25th July

No colony growth and hence no plasmid uptake. Transformation was repeated.

Vial Ampicillin Plate Plasmid Insertion Colony Count
1 (Main) Yes Yes 0
2 (Positive Control) No Yes 100+
3 (Negative Control) Yes No 0

Friday 26th July

Once again our cells were unsuccessful in taking up the YB3110 plasmid. We concluded that our batch of competent cells were not competent and so these will not be used in any further experiments. A new batch of competent cells are to be generated.

Vial Ampicillin Plate Plasmid Insertion Colony Count
1 (Main) Yes Yes 0
2 (Positive Control) No Yes 100+
3 (Negative Control) Yes No 0

Mammalian Lab

Monday 22nd July

MEF passage 6. Training ends.