Team:UCL/Project/Circuit

From 2013.igem.org

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MMP-9 is also known as gelatinase B, this is an enzyme encoded by the MMP9 gene in humans. Most commonly known for its role in breaking down the extracellular matrix. Naturally it is secreted in its inactive form and must be cleaved by other proteases, but our GEM are are meant to produce just the active form. It has been shown by Yan et al. that MMP-9 is the only known endogenous protease that degrades both fibrillar and soluble forms of amyloid-β peptide (Aβ). This satisfies the <a href="https://2013.igem.org/Team:UCL/Background/Neuropathology" target="_blank">'Amyloid Cascade Hypothesis'</a> as well as theories that see plaques as <a href="https://2013.igem.org/Team:UCL/Background/Neuropathology" target="_blank">neuroprotective</a>, and soluble Aβ as the real threat.  MMP-9 is expressed in specific cells at low basal levels and may keep plaque size in dynamic equilibrium <a href="http://www.ncbi.nlm.nih.gov/pubmed/16787929" target="_blank">(Yan et al. 2006)</a>. Over producing it in (inactive) GEM could greatly improve both soluble and insoluble Aβ clearance. MMP-9 must be delivered in GEM and expressed only in the vicinity of plaques, as otherwise it could cause damage to brain tissue if, for example, injected into the brain.
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<p class="major_title">VIP</p>
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Oxidative stress via free radical production increases with proximity to senile plaques <a href="http://www.ncbi.nlm.nih.gov/pubmed/10863548" target="_blank">(Colton et al., 2000)</a>. The microglia immune response around plaques also increases oxidative stress. Therefore, we have designed a promoter which will initiate transcription in response to oxidative stress to ensure the production of key proteins only in the plaques’ locales. This promoter is an improvement of a yeast minimal promoter (<a href="http://parts.igem.org/Part:BBa_K105027" target="_blank">cyc100</a>) already in the registry. NF-κB is a transcription factor which translocates to the nucleus under oxidative stress <a href="http://www.ncbi.nlm.nih.gov/pubmed/12730877" target="_blank">(Shi et al., 2003)</a>, and binds to the sequence GGGAATTT <a href="http://www.ncbi.nlm.nih.gov/pubmed/19435890" target="_blank">(Park et al., 2009)</a>. Thus, by placing this site upstream of the yeast minimal promoter, we created a novel mammalian promoter which initiates transcription in response to oxidative stress.
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<p class="major_title">BDNF</p>
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Oxidative stress via free radical production increases with proximity to senile plaques <a href="http://www.ncbi.nlm.nih.gov/pubmed/10863548" target="_blank">(Colton et al., 2000)</a>. The microglia immune response around plaques also increases oxidative stress. Therefore, we have designed a promoter which will initiate transcription in response to oxidative stress to ensure the production of key proteins only in the plaques’ locales. This promoter is an improvement of a yeast minimal promoter (<a href="http://parts.igem.org/Part:BBa_K105027" target="_blank">cyc100</a>) already in the registry. NF-κB is a transcription factor which translocates to the nucleus under oxidative stress <a href="http://www.ncbi.nlm.nih.gov/pubmed/12730877" target="_blank">(Shi et al., 2003)</a>, and binds to the sequence GGGAATTT <a href="http://www.ncbi.nlm.nih.gov/pubmed/19435890" target="_blank">(Park et al., 2009)</a>. Thus, by placing this site upstream of the yeast minimal promoter, we created a novel mammalian promoter which initiates transcription in response to oxidative stress.
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<p class="major_title">IP-10</p>
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Oxidative stress via free radical production increases with proximity to senile plaques <a href="http://www.ncbi.nlm.nih.gov/pubmed/10863548" target="_blank">(Colton et al., 2000)</a>. The microglia immune response around plaques also increases oxidative stress. Therefore, we have designed a promoter which will initiate transcription in response to oxidative stress to ensure the production of key proteins only in the plaques’ locales. This promoter is an improvement of a yeast minimal promoter (<a href="http://parts.igem.org/Part:BBa_K105027" target="_blank">cyc100</a>) already in the registry. NF-κB is a transcription factor which translocates to the nucleus under oxidative stress <a href="http://www.ncbi.nlm.nih.gov/pubmed/12730877" target="_blank">(Shi et al., 2003)</a>, and binds to the sequence GGGAATTT <a href="http://www.ncbi.nlm.nih.gov/pubmed/19435890" target="_blank">(Park et al., 2009)</a>. Thus, by placing this site upstream of the yeast minimal promoter, we created a novel mammalian promoter which initiates transcription in response to oxidative stress.
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Revision as of 16:10, 4 September 2013

CIRCUIT OVERVIEW

IGEM: Intelligently Genetically Engineered Microglia

Our ambitious project concerns bringing synthetic biology to the brain in order to try a novel approach to tackling Alzheimer’s Disease (AD). Microglia are mobile brain cells, making them an ideal chassis. To do this, our proposed treatment would involve extracting microglia from a patient, or using a specially bred immortalised line of human microglia, to avoid rejection, and transfecting it with our new genetic circuit. Implantation into the brain could be performed surgically or using a viral vector - but in order to better control the numbers of genetically engineered microglia (GEM) in the brain micro-neurosurgery may prove best. Our circuit is designed to detect detect amyloid plaques, attract other microglia, degrade the plaques, reduce neuroinflammation and support dying neurons. Theoretically, this should halt the progression of Alzheimer’s disease and could lead to other forms of neuro-genetic engineering.

Our GEM have zeocin resistance would be injected into the key areas of pathology in the AD brain; the neocortex, limbic structures, hippocampus, amygdala, and some of the brainstem nuclei. Microglia are naturally drawn towards the senile plaques characteristic of AD. Senile plaques create free radicals, so as the GEM near the plaques oxidative stress increases. In response, our oxidative stress promoter will increase the transcription of key genes. Their protein products are matrix metalloproteinase 9 (MMP-9) , vasoactive intestinal peptide(VIP), brain derived neurotrophic factor (BDNF) and a chemoattractant called interferon gamma-induced protein 10(IP-10).

Brain cell death in AD is engendered by neuroinflammation due to microglia activation. VIP is able to de-activate GEM and native microglia. Activated microglia do not, however, produce proteases to clear plaques or attract other microglia. MMP-9 is capable of degrading β-amyloid fibrils and soluble β-amyloid in situ (Yan et al. 2006). Many theories of AD causation see amyloid build up as key to pathogenesis. Increasing clearance could therefore halt disease progression, and synthetically expressing MMP-9 in volume will ensure greater than natural plaque degradation even after microglial inactivation. In order attract more GEM to increase clearance rate, the chemoattractant is produced. AD may also arise due to insiufficient BDNF signaling (Frade & Lopez-Sanchez 2010), and BDNF is able to support dying neurons, which is why we also propose its production.

Zeocin Resistance

Zeocin is a glycopeptide antibiotic capable of killing most bacteria, fungi, yeast, plant, and animal cells by intercalating DNA and inducing double strand breakage. This makes zeocin resistance an ideal selective maker for our project, which involves both bacterial and mammalian chassis. The product of the Sh ble gene, isolated from the bacterium Streptoalloteichus hindustanus (Gatignol et al. 1988), confers zeocin resistance to transfected/transformed cells. Sh ble is a small binding protein with strong affinity for antibiotics on a one to one ratio. It prevents zeocin from being activated by ferrous ions and oxygen, meaning it cannot react in vitro with DNA.

Oxidative Stress Promoter

Oxidative stress via free radical production increases with proximity to senile plaques (Colton et al., 2000). The microglia immune response around plaques also increases oxidative stress. Therefore, we have designed a promoter which will initiate transcription in response to oxidative stress to ensure the production of key proteins only in the plaques’ locales. This promoter is an improvement of a yeast minimal promoter (cyc100) already in the registry. NF-κB is a transcription factor which translocates to the nucleus under oxidative stress (Shi et al., 2003), and binds to the sequence GGGAATTT (Park et al., 2009). Thus, by placing this site upstream of the yeast minimal promoter, we created a novel mammalian promoter which initiates transcription in response to oxidative stress.

Active MMP-9

MMP-9 is also known as gelatinase B, this is an enzyme encoded by the MMP9 gene in humans. Most commonly known for its role in breaking down the extracellular matrix. Naturally it is secreted in its inactive form and must be cleaved by other proteases, but our GEM are are meant to produce just the active form. It has been shown by Yan et al. that MMP-9 is the only known endogenous protease that degrades both fibrillar and soluble forms of amyloid-β peptide (Aβ). This satisfies the 'Amyloid Cascade Hypothesis' as well as theories that see plaques as neuroprotective, and soluble Aβ as the real threat. MMP-9 is expressed in specific cells at low basal levels and may keep plaque size in dynamic equilibrium (Yan et al. 2006). Over producing it in (inactive) GEM could greatly improve both soluble and insoluble Aβ clearance. MMP-9 must be delivered in GEM and expressed only in the vicinity of plaques, as otherwise it could cause damage to brain tissue if, for example, injected into the brain.

VIP

Oxidative stress via free radical production increases with proximity to senile plaques (Colton et al., 2000). The microglia immune response around plaques also increases oxidative stress. Therefore, we have designed a promoter which will initiate transcription in response to oxidative stress to ensure the production of key proteins only in the plaques’ locales. This promoter is an improvement of a yeast minimal promoter (cyc100) already in the registry. NF-κB is a transcription factor which translocates to the nucleus under oxidative stress (Shi et al., 2003), and binds to the sequence GGGAATTT (Park et al., 2009). Thus, by placing this site upstream of the yeast minimal promoter, we created a novel mammalian promoter which initiates transcription in response to oxidative stress.

BDNF

Oxidative stress via free radical production increases with proximity to senile plaques (Colton et al., 2000). The microglia immune response around plaques also increases oxidative stress. Therefore, we have designed a promoter which will initiate transcription in response to oxidative stress to ensure the production of key proteins only in the plaques’ locales. This promoter is an improvement of a yeast minimal promoter (cyc100) already in the registry. NF-κB is a transcription factor which translocates to the nucleus under oxidative stress (Shi et al., 2003), and binds to the sequence GGGAATTT (Park et al., 2009). Thus, by placing this site upstream of the yeast minimal promoter, we created a novel mammalian promoter which initiates transcription in response to oxidative stress.

IP-10

Oxidative stress via free radical production increases with proximity to senile plaques (Colton et al., 2000). The microglia immune response around plaques also increases oxidative stress. Therefore, we have designed a promoter which will initiate transcription in response to oxidative stress to ensure the production of key proteins only in the plaques’ locales. This promoter is an improvement of a yeast minimal promoter (cyc100) already in the registry. NF-κB is a transcription factor which translocates to the nucleus under oxidative stress (Shi et al., 2003), and binds to the sequence GGGAATTT (Park et al., 2009). Thus, by placing this site upstream of the yeast minimal promoter, we created a novel mammalian promoter which initiates transcription in response to oxidative stress.