Team:UCSF/Project/Accomplish

From 2013.igem.org

(Difference between revisions)
(Created page with "{{Template:UCSF/MainHeader}}")
Line 1: Line 1:
{{Template:UCSF/MainHeader}}
{{Template:UCSF/MainHeader}}
 +
 +
 +
 +
<html>
 +
 +
 +
<link rel="stylesheet" type="text/css" href="https://2013.igem.org/Team:UCSF/stylesheet"/>
 +
<head>
 +
 +
 +
<style>
 +
 +
#mission {width: 500px; float:left; background-color: #F5F5F5; margin-left:8px; padding: 10px; margin-top:8px;}
 +
#opensource {width:306px; float:left; background-color: #F5F5F5; margin-left:8px; padding: 10px; margin-top:8px;}
 +
#rightcontent {width:755px; float:right; background-color: #F5F5F5; margin-left: 8px;  margin-top:10px;}
 +
#photos {width:950px;  background-color: #FFFFFF; margin-left: 8px;  margin-top:10px;}
 +
#movie {width:950px;  background-color: #FFFFFF; margin-left: 8px;}
 +
#description{width:950px; float:left; background-color: #FFFFFF; margin-left: 8px;  margin-top:10px;}
 +
#flickr{width:755px; float:right;} 
 +
#leftcolumntotal{width:200px; height:1800px; float: left; margin-top:0px;}
 +
#rightcontenttext {width:900px; float:right; background-color: #FFFFFF; margin-left:8px; padding: 10px; margin-top:8px;}
 +
 +
}
 +
 +
</style>
 +
 +
 +
<div id="description" align="justify" style = "width:950px">
 +
<font face="arial" size = "4">
 +
 +
<ul style="list-style-image:url(https://static.igem.org/mediawiki/2013/7/7b/IGEM_Logo_UCSF_Bullet.png);">
 +
 +
<li>We have shown that CRISPRi can be introduced to cells through bacterial conjugation. This is extremely exciting and provides the opportunity for gene expression to be altered in specific cells in a mixed population of bacteria. </li><br>
 +
 +
<li>We have created a standard for creating "knock-in" cassettes and inserting DNA into the <i>E. coli</i> genome. Using a combination of PCR sewing and lambda red recombination, we inserted two fluorescent proteins into the <i>E. coli</i> genome to mark our conjugation strains.  </li><br>

Revision as of 07:33, 27 September 2013


  • We have shown that CRISPRi can be introduced to cells through bacterial conjugation. This is extremely exciting and provides the opportunity for gene expression to be altered in specific cells in a mixed population of bacteria.

  • We have created a standard for creating "knock-in" cassettes and inserting DNA into the E. coli genome. Using a combination of PCR sewing and lambda red recombination, we inserted two fluorescent proteins into the E. coli genome to mark our conjugation strains.