http://2013.igem.org/wiki/index.php?title=Team:UCSF/Project/Accomplish&feed=atom&action=historyTeam:UCSF/Project/Accomplish - Revision history2024-03-29T14:52:40ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:UCSF/Project/Accomplish&diff=241913&oldid=prevVzepeda at 03:50, 28 September 20132013-09-28T03:50:47Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>We have shown that after being introduced by conjugation, our CRISPRi system is functional and capable of repressing a targeted gene (RFP). </li><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>We have shown that after being introduced by conjugation, our CRISPRi system is functional and capable of repressing a targeted gene (RFP). </li><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>We have created a standard for creating "knock-in" cassettes and inserting DNA into the <i>E. coli</i> genome. Using a combination of PCR sewing and lambda red recombination, we inserted two fluorescent proteins into the <i>E. coli</i> genome to mark our conjugation strains. Our protocol was tested several times and has proven to be very effective. </li><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>We have created a standard for creating "knock-in" cassettes and inserting DNA into the <i>E. coli</i> genome. Using a combination of PCR sewing and lambda red recombination, we inserted two fluorescent proteins into the <i>E. coli</i> genome to mark our conjugation strains. Our protocol was tested several times and has proven to be very effective. <ins class="diffchange diffchange-inline"><a href=https://dl.dropboxusercontent.com/u/24404809/iGEM%202013/Standard%20-%20Inserting%20Marker%20into%20Chromosome.doc>Write-up for our Standard</a></ins></li><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>We have created a set of materials that can be used to introduce synthetic biology to the public. These materials were produced as a part of our outreach and human practices projects and are available for download on our Materials page.</li><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>We have created a set of materials that can be used to introduce synthetic biology to the public. These materials were produced as a part of our outreach and human practices projects and are available for download on our Materials page.</li><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>We have further characterized the pTET and pLAC promoters that are widely used in the iGEM registry and placed this data on the parts registry pages.</li><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>We have further characterized the pTET and pLAC promoters that are widely used in the iGEM registry and placed this data on the parts registry pages.</li><br></div></td></tr>
</table>Vzepedahttp://2013.igem.org/wiki/index.php?title=Team:UCSF/Project/Accomplish&diff=236959&oldid=prevVzepeda at 02:41, 28 September 20132013-09-28T02:41:23Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul style="list-style-image:url(https://static.igem.org/mediawiki/2013/7/7b/IGEM_Logo_UCSF_Bullet.png);"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul style="list-style-image:url(https://static.igem.org/mediawiki/2013/7/7b/IGEM_Logo_UCSF_Bullet.png);"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>We have shown that CRISPRi can be introduced to cells through bacterial conjugation. This is <del class="diffchange diffchange-inline">extremely exciting </del>and provides the opportunity for <del class="diffchange diffchange-inline">gene expression to be altered in specific cells in a mixed population of bacteria</del>. </li><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>We have shown that CRISPRi can be introduced to cells through bacterial conjugation. This is <ins class="diffchange diffchange-inline">the first time that this has been shown </ins>and provides the opportunity for <ins class="diffchange diffchange-inline">many future applications</ins>. </li><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>We have created a standard for creating "knock-in" cassettes and inserting DNA into the <i>E. coli</i> genome. Using a combination of PCR sewing and lambda red recombination, we inserted two fluorescent proteins into the <i>E. coli</i> genome to mark our conjugation strains. <del class="diffchange diffchange-inline"> </del></li><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li>We have shown that after being introduced by conjugation, our CRISPRi system is functional and capable of repressing a targeted gene (RFP). </li><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>We have created a standard for creating "knock-in" cassettes and inserting DNA into the <i>E. coli</i> genome. Using a combination of PCR sewing and lambda red recombination, we inserted two fluorescent proteins into the <i>E. coli</i> genome to mark our conjugation strains. <ins class="diffchange diffchange-inline">Our protocol was tested several times and has proven to be very effective. </li><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li>We have created a set of materials that can be used to introduce synthetic biology to the public. These materials were produced as a part of our outreach and human practices projects and are available for download on our Materials page.</li><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><li>We have further characterized the pTET and pLAC promoters that are widely used in the iGEM registry and placed this data on the parts registry pages.</ins></li><br></div></td></tr>
</table>Vzepedahttp://2013.igem.org/wiki/index.php?title=Team:UCSF/Project/Accomplish&diff=234154&oldid=prevVhuang at 01:51, 28 September 20132013-09-28T01:51:57Z<p></p>
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</table>Vhuanghttp://2013.igem.org/wiki/index.php?title=Team:UCSF/Project/Accomplish&diff=200385&oldid=prevVzepeda at 07:33, 27 September 20132013-09-27T07:33:21Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><li>We have shown that CRISPRi can be introduced to cells through bacterial conjugation. This is extremely exciting and provides the opportunity for gene expression to be altered in specific cells in a mixed population of bacteria. </li><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><li>We have created a standard for creating "knock-in" cassettes and inserting DNA into the <i>E. coli</i> genome. Using a combination of PCR sewing and lambda red recombination, we inserted two fluorescent proteins into the <i>E. coli</i> genome to mark our conjugation strains. </li><br></ins></div></td></tr>
</table>Vzepedahttp://2013.igem.org/wiki/index.php?title=Team:UCSF/Project/Accomplish&diff=112155&oldid=prevVzepeda: Created page with "{{Template:UCSF/MainHeader}}"2013-09-09T23:04:08Z<p>Created page with "{{Template:UCSF/MainHeader}}"</p>
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