Team:UCSF/Project/Accomplish

From 2013.igem.org

Revision as of 01:51, 28 September 2013 by Vhuang (Talk | contribs)


  • We have shown that CRISPRi can be introduced to cells through bacterial conjugation. This is extremely exciting and provides the opportunity for gene expression to be altered in specific cells in a mixed population of bacteria.

  • We have created a standard for creating "knock-in" cassettes and inserting DNA into the E. coli genome. Using a combination of PCR sewing and lambda red recombination, we inserted two fluorescent proteins into the E. coli genome to mark our conjugation strains.