Team:UCSF/Project/Background2
From 2013.igem.org
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<p2>At the beginning of this summer, we asked ourselves a question: "What could we introduce to a microbiome which would allow targeting and eventual gene expression changes in a specific bacteria?" The difficulty faced with this situation is in:</p2> | <p2>At the beginning of this summer, we asked ourselves a question: "What could we introduce to a microbiome which would allow targeting and eventual gene expression changes in a specific bacteria?" The difficulty faced with this situation is in:</p2> | ||
<p2> 1. Introduce a targeting system into a defined mixture of bacteria such that you can select and introduce manipulations without negatively affecting other bacteria. </p2> | <p2> 1. Introduce a targeting system into a defined mixture of bacteria such that you can select and introduce manipulations without negatively affecting other bacteria. </p2> | ||
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<p2>WHY USE CRISPRi? <br><br>1. CRISPRi utilizes gRNAs which are highly specific and customizable.<br><br> | <p2>WHY USE CRISPRi? <br><br>1. CRISPRi utilizes gRNAs which are highly specific and customizable.<br><br> | ||
2. In principle it could be used to take advantage of unique DNA sequences to target specific bacterial species.</p2> | 2. In principle it could be used to take advantage of unique DNA sequences to target specific bacterial species.</p2> | ||
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<img style="height:400px; margin-left:50px; padding:0;"src="https://static.igem.org/mediawiki/2013/a/a6/CRISPRi_Background_UCSF-1.png"> | <img style="height:400px; margin-left:50px; padding:0;"src="https://static.igem.org/mediawiki/2013/a/a6/CRISPRi_Background_UCSF-1.png"> | ||
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- | <p3><center>Amended from: http://www.cell.com/abstract/S0092-8674(13)00211-0?script=true | + | <p3><center>Amended from: http://www.cell.com/abstract/S0092-8674(13)00211-0?script=true</center></p3> |
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Revision as of 08:54, 28 October 2013
Operation CRISPR: Deploying precision guided tools to target unique species in a complex microbiome
1. Introducing CRISPRi to a bacterial community
1. CRISPRi utilizes gRNAs which are highly specific and customizable.
2. In principle it could be used to take advantage of unique DNA sequences to target specific bacterial species.