Team:UCSF/Project/Background2
From 2013.igem.org
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<p2> As a means <a href="https://2013.igem.org/Team:UCSF/Project/Conjugation/Design" >to introduce our CRISPRi system into a microbial community we've opted to utilize conjugation </a> - a naturally occurring mechanism bacteria use to transfer DNA. By utilizing this mechanism, we are able to target specific strains of bacteria and affect gene expression. This will have a potential for future applications that require targeting individual strains in a bacterial community. </p2></div> | <p2> As a means <a href="https://2013.igem.org/Team:UCSF/Project/Conjugation/Design" >to introduce our CRISPRi system into a microbial community we've opted to utilize conjugation </a> - a naturally occurring mechanism bacteria use to transfer DNA. By utilizing this mechanism, we are able to target specific strains of bacteria and affect gene expression. This will have a potential for future applications that require targeting individual strains in a bacterial community. </p2></div> | ||
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src="https://static.igem.org/mediawiki/2013/8/8b/Conjugation_Bkgrd_UCSF-1.png"> </center><br></div> | src="https://static.igem.org/mediawiki/2013/8/8b/Conjugation_Bkgrd_UCSF-1.png"> </center><br></div> | ||
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+ | <p1><center>The combination of conjugation and CRISPRi allows us to create a system capable of both transferring genetic instructions from one cell to another as well as targeting unique species in a microbial community through a specific gene. </center></p1> | ||
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Revision as of 09:10, 28 October 2013
Operation CRISPR: Deploying precision guided tools to target unique species in a complex microbiome
1. Introducing CRISPRi to a bacterial community
1. CRISPRi utilizes gRNAs which are highly specific and customizable.
2. In principle it could be used to take advantage of unique DNA sequences to target specific bacterial species.