Team:UC Chile

From 2013.igem.org

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                         <ul class="sub oculto">
                         <ul class="sub oculto">
                             <li class="pos1 sub_item oculto">BioBricks<a href="https://2013.igem.org/Team:UC_Chile/BioBricks"></a></li>
                             <li class="pos1 sub_item oculto">BioBricks<a href="https://2013.igem.org/Team:UC_Chile/BioBricks"></a></li>
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                             <li class="pos1 sub_item oculto">Mathematical Model<a href="https://2013.igem.org/Team:UC_Chile/Mathematical Model"></a></li>
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                             <li class="pos2 sub_item oculto">Mathematical Model<a href="https://2013.igem.org/Team:UC_Chile/Mathematical Model"></a></li>
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                             <li class="pos1 sub_item oculto">Wet Lab<a href="https://2013.igem.org/Team:UC_Chile/Wet Lab"></a></li>                             
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                             <li class="pos3 sub_item oculto">Wet Lab<a href="https://2013.igem.org/Team:UC_Chile/Wet Lab"></a></li>                             
                         </ul>
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                         <ul class="sub oculto">
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                             <li class="pos1 sub_item oculto">Home<a href="https://2013.igem.org/Team:UC_Chile/Human Practices"></a></li>
                             <li class="pos1 sub_item oculto">Home<a href="https://2013.igem.org/Team:UC_Chile/Human Practices"></a></li>
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                             <li class="pos1 sub_item oculto">Enterpreuners<a href="https://2013.igem.org/Team:UC_Chile/Enterpreuners"></a></li>
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                             <li class="pos2 sub_item oculto">Synbio web<a href="https://2013.igem.org/Team:UC_Chile/Synbio web"></a></li>
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                            <li class="pos1 sub_item oculto">Game<a href="https://2013.igem.org/Team:UC_Chile/Game"></a></li>
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                            <li class="pos1 sub_item oculto">Frontier Seminar<a href="https://2013.igem.org/Team:UC_Chile/Frontier Seminar"></a></li>
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                            <li class="pos1 sub_item oculto">Synbio web<a href="https://2013.igem.org/Team:UC_Chile/Synbio web"></a></li>
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                            <li class="pos1 sub_item oculto">Ingenia<a href="https://2013.igem.org/Team:UC_Chile/Ingenia"></a></li>
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                         </ul>
                         </ul>
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                         <div class="text">Home</div>
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                         <div class="text">More</div>
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                         <ul class="sub oculto">
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                             <li class="pos1 sub_item ">Home<a href="https://2013.igem.org/Team:UC_Chile"></a></li>
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                             <li class="pos1 sub_item oculto">Acknowledgments<a href="https://2013.igem.org/Team:UC_Chile/Acknowledgments"></a></li>
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                             <li class="pos1 sub_item ">Overview<a href="https://2013.igem.org/Team:UC_Chile/Overview"></a></li>
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                             <li class="pos2 sub_item oculto">Biosafety<a href="https://2013.igem.org/Team:UC_Chile/Biosafety"></a></li>
                         </ul>
                         </ul>
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                             <li class="pos3 sub_item oculto">Targeting<a href="https://2013.igem.org/Team:UC_Chile/Targeting"></a></li>
                             <li class="pos3 sub_item oculto">Targeting<a href="https://2013.igem.org/Team:UC_Chile/Targeting"></a></li>
                             <li class="pos4 sub_item oculto">Purification<a href="https://2013.igem.org/Team:UC_Chile/Purification"></a></li>
                             <li class="pos4 sub_item oculto">Purification<a href="https://2013.igem.org/Team:UC_Chile/Purification"></a></li>
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                             <li class="pos4 sub_item oculto">Disruption<a href="https://2013.igem.org/Team:UC_Chile/Disruption"></a></li>
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                             <li class="pos5 sub_item oculto">Disruption<a href="https://2013.igem.org/Team:UC_Chile/Disruption"></a></li>
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                             <li class="pos4 sub_item oculto">In vitro chaneling<a href="https://2013.igem.org/Team:UC_Chile/In vitro chaneling"></a></li>
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                             <li class="pos5 sub_item oculto">In vitro Channeling<a href="https://2013.igem.org/Team:UC_Chile/In vitro Channeling"></a></li>                          
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                            <li class="pos4 sub_item oculto">Results<a href="https://2013.igem.org/Team:UC_Chile/Results"></a></li>
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                         </ul>
                         </ul>
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                         <div class="text">Methodology</div>
                         <div class="text">Methodology</div>
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                         <ul class="sub oculto">
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                             <li class="pos2 sub_item oculto">Lab Notebook<a href="https://2013.igem.org/Team:UC_Chile/Lab NoteBook"></a></li>
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                             <li class="pos1 sub_item oculto">Lab Notebook<a href="https://2013.igem.org/Team:UC_Chile/Lab NoteBook"></a></li>
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                             <li class="pos1 sub_item oculto">Protocols<a href="https://2013.igem.org/Team:UC_Chile/Protocols"></a></li>
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                             <li class="pos2 sub_item oculto">Protocols<a href="https://2013.igem.org/Team:UC_Chile/Protocols"></a></li>
                         </ul>
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                         <div class="text">More</div>
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                         <div class="text">Home</div>
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                         <ul class="sub oculto">
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                            <li class="pos3 sub_item oculto">Acknowledgments<a href="https://2013.igem.org/Team:UC_Chile/Acknowledgments"></a></li>
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                             <li class="pos1 sub_item oculto">Overview<a href="https://2013.igem.org/Team:UC_Chile/Overview"></a></li>
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                            <li class="pos2 sub_item oculto">Judging Criteria<a href="https://2013.igem.org/Team:UC_Chile/Judging Criteria"></a></li>
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                             <li class="pos1 sub_item oculto">Biosafety<a href="https://2013.igem.org/Team:UC_Chile/Biosafety"></a></li>
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    <h1>Overview</h1>
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Imagine a world where production of different compound could be easily achievable, where industrial problems associated with genetic manipulation are over: there is no more instability in the energy flux of bacteria. Imagine a world where there are no more industrial biosafety issues because recombinant bacteria is no longer needed in situ. This is the world we propose: the world of in vitro production.
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To achieve this, we reengineered the Carboxysome, a bacterial microcompartment (BMC) to use it as a continuous reactor capable of generating any output by having the needed enzyme in its inner space. Make Carboxysome your own synthetic space for the production of anything you want:
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A Whateversisome!
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We have successfully:
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<ul>
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    <li>Characterized the Carboxysome as a tool for Synthetic Biology: we presented the plasmid as a standardized biobrick.</li>
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    <li>Found a targeting sequence to the inside of Carboxysome.</li>
 +
    <li>Designed an innovative methodology for purification and isolation of BMC.</li>
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    <li>We analyzed the potential combinatorial of Carboxysomes on in vitro channeling.</li>
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    </ul><br>       
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Even more…
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    <ul>
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    <li> We are working towards generating step-by-step in vitro reactions.</li>
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    </ul>
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Latest revision as of 23:59, 7 October 2013

Wiki-IGEM

Overview

Imagine a world where production of different compound could be easily achievable, where industrial problems associated with genetic manipulation are over: there is no more instability in the energy flux of bacteria. Imagine a world where there are no more industrial biosafety issues because recombinant bacteria is no longer needed in situ. This is the world we propose: the world of in vitro production. To achieve this, we reengineered the Carboxysome, a bacterial microcompartment (BMC) to use it as a continuous reactor capable of generating any output by having the needed enzyme in its inner space. Make Carboxysome your own synthetic space for the production of anything you want: A Whateversisome!
We have successfully:
  • Characterized the Carboxysome as a tool for Synthetic Biology: we presented the plasmid as a standardized biobrick.
  • Found a targeting sequence to the inside of Carboxysome.
  • Designed an innovative methodology for purification and isolation of BMC.
  • We analyzed the potential combinatorial of Carboxysomes on in vitro channeling.

Even more…
  • We are working towards generating step-by-step in vitro reactions.