Team:UChicago/Protocols

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Notebook > Protocols

Protocols page


Contents

General Lab Best Practices

Lab Notebook Best Practices (read: do these best practices)

Steps to 3A Assembly & Labeling Guidelines

Resuspending DNA from iGEM Kit Plates

Recipes

Making Agar Plates

Pouring the Plates

Making LB (500mL)

Agar Stab Protocols

Ligation Protocol

Transformation Procedures

Making Overnight Cultures

Miniprep Protocols

Making an agarose gel for gel purification

Enzymatic digestion

Gel extraction with the QIAGEN kit