http://2013.igem.org/wiki/index.php?title=Team:UGA-Georgia/Results&feed=atom&action=historyTeam:UGA-Georgia/Results - Revision history2024-03-28T21:14:41ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:UGA-Georgia/Results&diff=242196&oldid=prevSmithpa: /* BioBrick Tools */2013-09-28T03:55:40Z<p><span class="autocomment">BioBrick Tools</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Conclusion:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Conclusion:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>We have shown that our tool fluoresces around 3 times more than <del class="diffchange diffchange-inline">normal </del>Methanococcus making it a valuable tool for quantifying and regulating gene expression. We hope that future researchers and iGEM teams will use this tool in the future to use methanogens as an organism for synthetic biology.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>We have shown that our tool fluoresces around 3 times more than <ins class="diffchange diffchange-inline">wild type </ins>Methanococcus making it a valuable tool for quantifying and regulating gene expression. We hope that future researchers and iGEM teams will use this tool in the future to use methanogens as an organism for synthetic biology.</p></div></td></tr>
</table>Smithpahttp://2013.igem.org/wiki/index.php?title=Team:UGA-Georgia/Results&diff=216498&oldid=prevSmithpa: /* Geraniol Production */2013-09-27T18:56:36Z<p><span class="autocomment">Geraniol Production</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Conclusion:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Conclusion:</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We created geraniol standards of different concentrations and plotted the concentration of the standard against the integral area of the peak formed in the respective chromatograph. From here we were able to draw a regression line through the data points and obtain a linear function. We used this linear function to accurately calculate the amount of geraniol produced in our samples. So, we can conclude the production of geraniol as over 0.2% of the DLW in Methanococcus.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We created geraniol standards of different concentrations and plotted the concentration of the standard against the integral area of the peak formed in the respective chromatograph. From here we were able to draw a regression line through the data points and obtain a linear function. We used this linear function to accurately calculate the amount of geraniol produced in our samples. So, we can conclude the production of geraniol as over 0.2% of the DLW in Methanococcus <ins class="diffchange diffchange-inline">using the pAW42-GS (BBa-k1138000) model</ins>.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= BioBrick Tools =</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>= BioBrick Tools =</div></td></tr>
</table>Smithpahttp://2013.igem.org/wiki/index.php?title=Team:UGA-Georgia/Results&diff=215381&oldid=prevMwestb1: /* BioBrick Tools */2013-09-27T18:23:12Z<p><span class="autocomment">BioBrick Tools</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This graph shows the fluorescence of BBa-k1138002 in comparison to the Methanococcus wild type strain s0001. We report the fluorescence in our BioBrick part roughly as a 3 fold increase. Note that the wild type gives a small fluorescence reading due to the fact that Methanococcus is naturally bio-luminescent.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This graph shows the fluorescence of BBa-k1138002 in comparison to the Methanococcus wild type strain s0001. We report the fluorescence in our BioBrick part roughly as a 3 fold increase. Note that the wild type gives a small fluorescence reading due to the fact that Methanococcus is naturally bio-luminescent.</div></td></tr>
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</table>Mwestb1http://2013.igem.org/wiki/index.php?title=Team:UGA-Georgia/Results&diff=215367&oldid=prevMwestb1: /* BioBrick Tools */2013-09-27T18:22:41Z<p><span class="autocomment">BioBrick Tools</span></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">These are the two tools that we created that will allow you to regulate and quantify gene expression. One has an RBS and the other one does not. The one with the RBS can be used as a control and the one without can be used to insert your gene of interest to quantify expression. Both tools have EcoRI, NotI, XbaI, and NsiI restriction sites for insertion of a gene.</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">This graph shows the fluorescence of BBa-k1138002 in comparison to the Methanococcus wild type strain s0001. We report the fluorescence in our BioBrick part roughly as a 3 fold increase. Note that the wild type gives a small fluorescence reading due to the fact that Methanococcus is naturally bio-luminescent.</ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Conclusion:</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p></ins>We <ins class="diffchange diffchange-inline">have shown that </ins>our <ins class="diffchange diffchange-inline">tool fluoresces around 3 times more than normal Methanococcus making it </ins>a <ins class="diffchange diffchange-inline">valuable tool for quantifying and regulating gene expression</ins>. <ins class="diffchange diffchange-inline">We hope </ins>that <ins class="diffchange diffchange-inline">future researchers and iGEM teams will use this tool in </ins>the <ins class="diffchange diffchange-inline">future </ins>to <ins class="diffchange diffchange-inline">use methanogens as an organism for synthetic biology</ins>.<ins class="diffchange diffchange-inline"></p></ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">This graph shows the fluorescence of BBa-k1138002 in comparison to the Methanococcus wild type strain s0001. </del>We <del class="diffchange diffchange-inline">report the fluorescence in </del>our <del class="diffchange diffchange-inline">BioBrick part roughly as </del>a <del class="diffchange diffchange-inline">2</del>.<del class="diffchange diffchange-inline">5 fold increase. Note </del>that the <del class="diffchange diffchange-inline">wild type gives a small fluorescence reading due </del>to <del class="diffchange diffchange-inline">the fact that Methanococcus is naturally bio-luminescent</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
</table>Mwestb1http://2013.igem.org/wiki/index.php?title=Team:UGA-Georgia/Results&diff=175691&oldid=prevMwestb1: /* Geraniol Production */2013-09-25T23:22:21Z<p><span class="autocomment">Geraniol Production</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(4) This Mass Spec analysis of the previous sample recognizes the significant peak at 7.81 minutes as geraniol (aka trans-3,7-dimethyl-2,7-octadien-1-ol)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(4) This Mass Spec analysis of the previous sample recognizes the significant peak at 7.81 minutes as geraniol (aka trans-3,7-dimethyl-2,7-octadien-1-ol)</div></td></tr>
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</table>Mwestb1http://2013.igem.org/wiki/index.php?title=Team:UGA-Georgia/Results&diff=175688&oldid=prevMwestb1: /* Geraniol Production */2013-09-25T23:22:02Z<p><span class="autocomment">Geraniol Production</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(4) This Mass Spec analysis of the previous sample recognizes the significant peak at 7.81 minutes as geraniol (aka trans-3,7-dimethyl-2,7-octadien-1-ol)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(4) This Mass Spec analysis of the previous sample recognizes the significant peak at 7.81 minutes as geraniol (aka trans-3,7-dimethyl-2,7-octadien-1-ol)</div></td></tr>
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</table>Mwestb1http://2013.igem.org/wiki/index.php?title=Team:UGA-Georgia/Results&diff=175683&oldid=prevMwestb1: /* Geraniol Production */2013-09-25T23:21:21Z<p><span class="autocomment">Geraniol Production</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(4) This Mass Spec analysis of the previous sample recognizes the significant peak at 7.81 minutes as geraniol (aka trans-3,7-dimethyl-2,7-octadien-1-ol)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(4) This Mass Spec analysis of the previous sample recognizes the significant peak at 7.81 minutes as geraniol (aka trans-3,7-dimethyl-2,7-octadien-1-ol)</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(6) This chromatograph is of a pAW42-GS sample that we extracted geraniol from. The significant peak here with similar retention time to the standard was confirmed as geraniol via Mass Spec (see window in bottom corner of graph)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(6) This chromatograph is of a pAW42-GS sample that we extracted geraniol from. The significant peak here with similar retention time to the standard was confirmed as geraniol via Mass Spec (see window in bottom corner of graph)</div></td></tr>
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</table>Mwestb1http://2013.igem.org/wiki/index.php?title=Team:UGA-Georgia/Results&diff=175091&oldid=prevMwestb1: /* Geraniol Production */2013-09-25T21:59:12Z<p><span class="autocomment">Geraniol Production</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(6) This chromatograph is of a pAW42-GS sample that we extracted geraniol from. The significant peak here with similar retention time to the standard was confirmed as geraniol via Mass Spec (see window in bottom corner of graph)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>(6) This chromatograph is of a pAW42-GS sample that we extracted geraniol from. The significant peak here with similar retention time to the standard was confirmed as geraniol via Mass Spec (see window in bottom corner of graph)</div></td></tr>
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</table>Mwestb1http://2013.igem.org/wiki/index.php?title=Team:UGA-Georgia/Results&diff=175085&oldid=prevMwestb1: /* Geraniol Production */2013-09-25T21:58:32Z<p><span class="autocomment">Geraniol Production</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">(3) This chromatograph is of another one of our samples. The significant peak with similar retention time to the standard is geraniol. This was confirmed using Mass Spec (see image below).</del></div></td><td colspan="2"> </td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">(3) This chromatograph is of another one of our samples. The significant peak with similar retention time to the standard is geraniol. This was confirmed using Mass Spec (see image below).</ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">(4) This Mass Spec analysis of the previous sample recognizes the significant peak at 7.81 minutes as geraniol (aka trans-3,7-dimethyl-2,7-octadien-1-ol)</del></div></td><td colspan="2"> </td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">(4) This Mass Spec analysis of the previous sample recognizes the significant peak at 7.81 minutes as geraniol (aka trans-3,7-dimethyl-2,7-octadien-1-ol)</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">(5) This chromatograph is of a geraniol standard we created and ran on 09/06/2013. The significant peak here was confirmed via Mass Spec as geraniol (see window in top corner of image).</ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">(5) This chromatograph is of a geraniol standard we created and ran on 09/06/2013. The significant peak here was confirmed via Mass Spec as geraniol (see window in top corner of image).</del></div></td><td colspan="2"> </td></tr>
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</table>Mwestb1http://2013.igem.org/wiki/index.php?title=Team:UGA-Georgia/Results&diff=175065&oldid=prevSmithpa: /* BioBrick Tools */2013-09-25T21:56:52Z<p><span class="autocomment">BioBrick Tools</span></p>
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