Team:UNITN-Trento

From 2013.igem.org

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             <p><i>B. fruity</i> envisions a new environmental friendly way to control fruit ripening by exploiting an engineered, light-regulated strain of <i>B. subtilis</i>. The system works by synthesizing ethylene or methyl salicylate (MeSA) upon photoinduction.</p>
             <p><i>B. fruity</i> envisions a new environmental friendly way to control fruit ripening by exploiting an engineered, light-regulated strain of <i>B. subtilis</i>. The system works by synthesizing ethylene or methyl salicylate (MeSA) upon photoinduction.</p>
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             <p><b>Ethylene</b> is a plant hormone widely used to ripen fruit. However its synthesis, handling, and storage is expensive and dangerous. In contrast, <i>B. fruity</i> produces ethylene from inexpensive material by hijacking a metabolic intermediate: 2-oxoglutarate. Our system converts this metabolite to ethylene through the activity of an ethylene forming enzyme (EFE) from <i>Pseudomonas syringae</i>.</p>
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             <p><b>Ethylene</b> is a plant hormone widely used to ripen fruit. However its synthesis, handling and storage is expensive and dangerous. In contrast, <i>B. fruity</i> produces ethylene from inexpensive material by a metabolic intermediate: 2-oxoglutarate. Our system converts this metabolite to ethylene through the activity of an ethylene forming enzyme (EFE) from <i>Pseudomonas syringae</i>.</p>
             <p><i>B. fruity</i> does not just accelerate ripening, but can also slow the process down, when desired, through the incorporation of a <b>methyl salicylate</b> (MeSA) synthesis pathway. MeSA was previously shown to inhibit the ripening of kiwifruit and tomatoes. The explored MeSA pathway builds upon the 2006 MIT iGEM project “<i>Eau de coli</i>”.</p>
             <p><i>B. fruity</i> does not just accelerate ripening, but can also slow the process down, when desired, through the incorporation of a <b>methyl salicylate</b> (MeSA) synthesis pathway. MeSA was previously shown to inhibit the ripening of kiwifruit and tomatoes. The explored MeSA pathway builds upon the 2006 MIT iGEM project “<i>Eau de coli</i>”.</p>

Revision as of 17:37, 2 October 2013


Achievements
  • Built and submitted 16 New BioBricks;
  • Successfully characterized 9 BioBricks;
  • Improved 4 parts;
  • Hosted a high school student in the lab for the summer;
  • Asked the opinion of local fruit consumers on our project;
  • Designed a fruit Vending Machine and a Home Edition B. fruity market product;
  • Ripened 5 bananas, 5 tomatoes, 3 kiwis, 2 plums, 2 apples;
  • Eaten one banana bread, one plums crumble and one apple strudel.