Team:UNITN-Trento/Notebook/Labposts/06/05

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{
{
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"date" : "2013-06-05",
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"date" : "2013-06-06",
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"author" : "viola",
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"author" : "caterina-michele-fabio",
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"title" : "transformation buffer",
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"title" : "We have the parts!",
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"content" : "<html>I made the transformation buffer for the competent cells that we will do tomorrow, 10mM tris HCl and 50 mM CaCl2. </html>",
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"content" : "<html>We performed the purification of <b>BBa_J45320</b>, <b>BBa_J45119</b>, <b>BBa_J45700</b> (obtained from NEB10&beta; colonies transformed by Viola, Emil and Pedro last week) following the “Wizard® Plus SV Minipreps DNA purification System” protocol. After the quantification through NanoDrop, we digested the plasmid with EcoR I and Pst I to obtain the devices. To verify the correct results of our purification we did an electrophoresis run as it is shown in the image. </html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/f/f2/Tn-2013-Digestion_EcoRI_PstI.jpg\" style =\"width: 450px\"></center></html>}}<html><br/></html>",
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"tags" : "competent cells"
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"tags" : "PchB-PchBA-BSMT1"
}
}

Latest revision as of 09:33, 3 October 2013

{ "date" : "2013-06-06", "author" : "caterina-michele-fabio", "title" : "We have the parts!", "content" : "We performed the purification of BBa_J45320, BBa_J45119, BBa_J45700 (obtained from NEB10β colonies transformed by Viola, Emil and Pedro last week) following the “Wizard® Plus SV Minipreps DNA purification System” protocol. After the quantification through NanoDrop, we digested the plasmid with EcoR I and Pst I to obtain the devices. To verify the correct results of our purification we did an electrophoresis run as it is shown in the image.

Gel Image

", "tags" : "PchB-PchBA-BSMT1" }

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