Team:UNITN-Trento/Notebook/Labposts/06/16

From 2013.igem.org

(Difference between revisions)
 
Line 1: Line 1:
{
{
-
"date" : "2013-06-07",
+
"date" : "2013-06-12",
-
"author" : "bruno-thomas",
+
"author" : "emil-fabio",
-
"title" : "Plates with ampicillin and chloramphenicol",
+
"title" : "Purification and verification of three Bacillus backbonesg",
-
"content" : "<html>We have prepared LB Agar and then added antibiotic. Finally we have plated the preparation in plastes. <br/>We have prepared plate at the concentration of 50 um/ml for ampicillin and 150 ul/ml for chloramphenicol.<br/><i>Seems that the concentration of Chloramphenicol is 5 times more concentrated than normally used.</i></html>",
+
"content" : "We have done the miniprep of the three backbone exploiting the Kit from Promega.N.B.Some sample have shown an unexpected red color. There is the possibilities that some backbones contain the RFP, maybe all. We have quantified the products with the following results:BBa_K823023(4 sample):220 ng/<html>&mu;</html>l , 228.9 ng/<html>&mu;</html>l ,246.4 ng/<html>&mu;</html>l , 272.3 ng/<html>&mu;</html>lBBa_K823022(3 sample): 222.8 ng/<html>&mu;</html>l , 227.5 ng/<html>&mu;</html>l ,284.9 ng/<html>&mu;</html>lBBa_K823027(2 sample):268 ng/<html>&mu;</html>l , 299.3 ng/<html>&mu;</html>lThen we have tried to verify the parts digesting with EcoR1 and Pst1 and doing an electrophoresis, unfortunately it seems that we didn't load the marker Fermentas 1 KB.",
-
"tags" : "mix"
+
"tags" : "pSBBs1C-pSBBs4S-pSBBs2E"
}
}

Latest revision as of 07:42, 3 October 2013

{ "date" : "2013-06-12", "author" : "emil-fabio", "title" : "Purification and verification of three Bacillus backbonesg", "content" : "We have done the miniprep of the three backbone exploiting the Kit from Promega.N.B.Some sample have shown an unexpected red color. There is the possibilities that some backbones contain the RFP, maybe all. We have quantified the products with the following results:BBa_K823023(4 sample):220 ng/μl , 228.9 ng/μl ,246.4 ng/μl , 272.3 ng/μlBBa_K823022(3 sample): 222.8 ng/μl , 227.5 ng/μl ,284.9 ng/μlBBa_K823027(2 sample):268 ng/μl , 299.3 ng/μlThen we have tried to verify the parts digesting with EcoR1 and Pst1 and doing an electrophoresis, unfortunately it seems that we didn't load the marker Fermentas 1 KB.", "tags" : "pSBBs1C-pSBBs4S-pSBBs2E" }

Retrieved from "http://2013.igem.org/Team:UNITN-Trento/Notebook/Labposts/06/16"