Team:UNITN-Trento/Notebook/Labposts/06/17

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(Created page with "{ "date" : "2013-06-14", "author" : "thomas", "title" : "EFE extraction and transformation Test!", "content" : "Since we received the Ethylen Forming Enzyme gene from Genescr...")
 
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{
{
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"date" : "2013-06-14",
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"date" : "2013-06-12",
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"author" : "thomas",
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"author" : "caterina-michele",
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"title" : "EFE extraction and transformation Test!",
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"title" : "miniprep SAMsynthetase+pSB1C3!",
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"content" : "Since we received the Ethylen Forming Enzyme gene from Genescript company, we proceeded with the extraction and the transformation test.We resuspended the 4 ug of DNA in 40 ul of sterilized water (obtaining a 100 ng/ul stock solution). After that, we transformed 200 ul of NEB10 competent cells with 1ul of EFE DNA.Finally we plated them in two 50 ug/ml Amp LB-Agar Petri dishes.'''Results:'''{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plate Image|<html><img id=\"post_img\" src=\"https://static.igem.org/mediawiki/2013/4/42/Tn-20130614-plate_TP.JPG\" width=\"500px\"/></html>}}As you can see from the image, we obtained many colonies. We then inculated x5 colonies in 5ml LB with Ampicillin.",
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"content" : "We took the three inocula of <b>SAMsynthetase+pSB1C3</b> and we performed the purification (Wizard Plus SV Miniprep DNA purification system). Then to verify if the colonies that we took contain the correct insert we perform the screening protocol and the gel eletrophoresis.",
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"tags" : "EFE"
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"tags" : "SAMsynthetase"
}
}

Latest revision as of 07:43, 3 October 2013

{ "date" : "2013-06-12", "author" : "caterina-michele", "title" : "miniprep SAMsynthetase+pSB1C3!", "content" : "We took the three inocula of SAMsynthetase+pSB1C3 and we performed the purification (Wizard Plus SV Miniprep DNA purification system). Then to verify if the colonies that we took contain the correct insert we perform the screening protocol and the gel eletrophoresis.", "tags" : "SAMsynthetase" }

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