From 2013.igem.org
(Difference between revisions)
|
|
| (5 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| | { | | { |
| - | "date" : "2013-06-07", | + | "date" : "2013-06-13", |
| - | "author" : "thomas-emil" | + | "author" : "bruno-fabio", |
| - | "title" : "Second SAMsynthase extraction attempt - TOO SAD", | + | "title" : "All day...all night...antibiotic plates", |
| - | "content" : "Since we didn't obtained our product using the Phusion PCR, we tried again to amplify the SAMsynthase gene from an extract of E.coli genomic DNA (strain MG1655). In order to do that we exploited the same primers used in our previous attempt (see 06-06-2013). | + | "content" : "<html>We wanted to do a trasformation but...we don't have the plates. So, at a good pace, we started to make plates with ampicillin and chloramphenicol.</html>", |
| - | | + | "tags" : "mix" |
| - | For the protocol used see the <html><a href="https://2013.igem.org/Team:UNITN-Trento/Protocols#RBC-Taq-DNA-pol-PCR">RBC Taq PCR protocol</a></html>..
| + | |
| - | When the reaction finished, we tested the presence of the aplificate product througt an electrophoresis analisys (adding 2 µl of LD for 10 µl of DNA).
| + | |
| - | | + | |
| - | <B>Results:</B> {{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/d/df/Tn-20130607-SAMsynth_taq.jpg" />
| + | |
| - | </html>}}
| + | |
| - | | + | |
| - | As you can see from our gel image, our product is present in both reactions (TAQ and TAQ+Phusion).
| + | |
| - | After purifying our products we find out that the concentration of DNA that we obtained is too low (about 5ng/ul) maybe for an experimental error in setting the PCR program. For this reason, our team is going to redo the PCR reaction again.
| + | |
| - | ", | + | |
| - | "tags" : "SAMsynthetase" | + | |
| | } | | } |
Latest revision as of 07:46, 3 October 2013
{
"date" : "2013-06-13",
"author" : "bruno-fabio",
"title" : "All day...all night...antibiotic plates",
"content" : "We wanted to do a trasformation but...we don't have the plates. So, at a good pace, we started to make plates with ampicillin and chloramphenicol.",
"tags" : "mix"
}
"
